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Lethal Heat Survival Assay

Fig. S1. Lethal Heat Survival Assay

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Lethal Heat Survival Assay

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  1. Fig. S1 Lethal Heat Survival Assay Strains were grown to mid-logarithmic phase (5 x 106 cells per ml) in YEPD medium, washed in PBS, and concentrated to 2 x 108 cells per ml. Aliquots of 100 μI were prepared in Eppendorf tubes. Then cells were heated at 50°C in water bath for 60 minutes. Tubes were removed and immediately placed on ice. The aliquots were then diluted accordingly and plated onto YEPD plates. Colonies were counted after 3 days of incubation at 30°C, and the percent viability was calculated with respect to cells not heated.

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