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Federal Centre of Breeding Research on Cultivated Plants

Federal Centre of Breeding Research on Cultivated Plants. Logistics in the project Christoph U.Germeier. WP2: Working Collection. P1. P5. P0. ELISA. PCR. P10. P6. P8. P9. Multipliers. Provide some germplasm. P15. P6. P8. seed. WP4: Fusarium Field Experiments.

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Federal Centre of Breeding Research on Cultivated Plants

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  1. Federal Centre of Breeding Research on Cultivated Plants • Logistics in the project • Christoph U.Germeier

  2. WP2: Working Collection P1 P5 P0 ELISA PCR P10 P6 P8 P9 Multipliers Provide some germplasm P15 P6 P8 seed WP4: Fusarium Field Experiments WP9: Frost Tolerance WP3: Quality Field Experiments WP5: Mycotoxin - Fusarium Field- Test P3 P14 P9 P8 P2 P1 P5 P8 P9 P10 P1 P0 P0 P8 P5 P6 P3 P7 P9 P11 P8 P9 Nordic (N) E-Europe SE (N) E SW S (S) E S S-Europe SE NIRS P11 P5 Climate C. P8 NP-HPLC Kalibration WP6: Protein and Fat WP1 Coordination P6 Enzyme P11 P9 P2 P6 Protein Fat WP8: Antioxidants AVA FIA Starch Fibre LC-MS/MS P7 WP7: ß-Glucan/Carbohydrates P12 P4 P15 WP10: Data Management P1 P2 P3 P4 P5 P6 P7 P9 P10 P11 P12 P14 P15 P8 seed seed samples samples samples

  3. Logisitics in the project 1.: Logistics in initial seed production (WP2 -> WP3,WP4, WP9 WP2: P5 -> WP6: Avenin WP2 -> WP9 2.: Logistics in quality analysis (WP3 -> WP6, WP7, WP8) 3.: Logistics in Fusarium and mycotoxin analysis (WP4 -> WP5 WP4 -> WP8?)

  4. Logistics in initial seed production WP2: Working Collection P1 P5 P0 P10 P6 P8 P9 Multipliers Provide some germplasm WP4: Fusarium Field Experiments WP3: Quality Field Experiments a) central distribution? P3 P14 P9 P8 P2 P1 P5 P8 P9 P10 P11 central distributor WP6: Avenin ? Nordic (N) E-Europe SE (N) E SW S (S) E S WP9: Frost Tolerance Field- Test P8 P9 S-Europe SE Climate C. P8 2007 2008 2008 2009

  5. Logistics in initial seed production WP2: Working Collection P1 P5 P0 P10 P6 P8 P9 Multipliers Provide some germplasm WP4: Fusarium Field Experiments WP3: Quality Field Experiments P3 P14 P9 P8 P2 P1 P5 P8 P9 P10 P11 WP6: Avenin Nordic (N) E-Europe SE (N) E SW S (S) E S WP9: Frost Tolerance Field- Test P8 P9 S-Europe SE Climate C. P8 b) partner by partner distribution? 2007 2008 2008 2009

  6. Logistics in quality analysis WP3: Quality Field Experiments P2 P1 P5 P8 P9 P10 P11 Nordic (N) E SW S (S) E NIRS Enzyme *verification NP-HPLC P5 P11 P6 P2 P6 WP6: Protein and Fat WP8: Antioxidants WP7: ß-Glucan/Carbohydrates Kalibration P12 P6 AVA P5 P11 P9 FIA Starch Fibre Protein Fat Minerals P7 2008 2009

  7. Logistics in quality analysis WP3: Quality Field Experiments P2 P1 P5 P8 P9 P10 P11 Nordic (N) E SW S (S) E *verification NIRS Enzyme NP-HPLC P5 P11 P6 P2 P6 WP6: Protein and Fat WP8: Antioxidants WP7: ß-Glucan/Carbohydrates Kalibration P12 P6 AVA P5 P11 P9 FIA Starch Fibre Protein Fat Minerals P7 2009 2010

  8. Summary: needed sample size in quality analysis 2008 2009 From field to lab From lab to lab

  9. Logistics in Fusarium and mycotoxin analysis WP4: Fusarium Field Experiments contrasting samples to one lab? P3 P14 P9 P8 similar samples to one lab? (N) E-Europe SE S NP-HPLC P6 ELISA PCR P15 P6 P8 WP8: Antioxidants *HT-2 and T-2, nivalenol, deoxynivalenol, zearalenone, diacetoxyscirpenol, 3- and 15-acetyldeoxynivalenol WP5: Mycotoxin - Fusarium AVA P7 LC-MS/MS P4 2008 2009

  10. Summary: needed sample size in Fusarium analysis From field to lab From lab to lab

  11. Thanks for your attention

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