Maternal deprivation induces a rapid decline in circulating leptin levels and sexually dimorphic modifications in hypoth

1. Maternal deprivation induces a rapid decline in circulating leptin levels and sexually dimorphic modifications in hypothalamic trophic factors and cell turnoverViveros et. al. Kristi Tschetter 2/12/10

2. Overview of Chiaruttini et. al. Coding region common to all BDNF transcipts that contains a constitutively active dendritic targeting signal Targeting signal is suppressed in transcripts containing exons 1 or 4 these are resistricted to the soma and proximal dendrites Targeting signal is mediated by translin (RNA-binding protein implicated in RNA trafficking) Translin binding is disrupted by the G196A (or Val66Met mutation) G196A mutation blocks dendritic targeting of BDNF mRNA by disrupting its interaction with translin

3. BDNF Transcipts Multiple BDNF transcripts are generated by alternative splicing on one 5� exon with a shared 3� exon containing the entire BDNF coding region and either a long or a short 3� UTR sequence Exon 1 and 4 transcripts are localized in the cell soma Exon 2 and 6 transcripts show a somatodendritic localization Splice variants appear to encode spatial localization signals used to preferentially regulate BDNF expression in different subcellular domains

4. Overall Translin mediates the constitutive dendritic targeting signal located in the CDS and this targeting mechanism is conserved in rat and human BDNF mRNA Evidence that translin/trax complex mediates dendritic targeting of BDNF mRNA BDNF coding region common to all BDNF slice variants is sufficient to direct constitutive targeting of BDNF mRNAs to the distal dendritic compartment

5. Figure 6

6. Figure 3