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Low. Normal. High. Intensity Spreading. Nuc. Displacement. F-actin. 1/(Form factor). Intensity CV. IxA (N+C). DMSO + anti-LFA-1. A. T. A. T. A. T. A. T. A. T. H-89 3. Go6976 3. Go6976 2. C. Staurosporine 3. Calyculin A 3. Staurosporine 2. Genistein 1. -Tubulin.

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Low

Normal

High

Intensity Spreading

Nuc. Displacement

F-actin

1/(Form factor)

Intensity CV

IxA (N+C)

DMSO + anti-LFA-1

A

T

A

T

A

T

A

T

A

T

H-89 3

Go6976 3

Go6976 2

C

Staurosporine 3

Calyculin A 3

Staurosporine 2

Genistein 1

-Tubulin

UCN-01 1

Bisindolymaleimide 2

U0126 3

Wortmannin 1

DMSO + anti-LFA-1

Wortmannin 2

Wortmannin 3

LY294002 3

LY294002 1

LY294002 2

Rottlerin 1

PD95089 2

PD95089 3

U0126 2

B

U0126 1

PD95089 1

SB203580 2

Cyclosporine 1

F-actin

Cyclosporine 2

Cyclosporine 3

SB203580 1

DMSO

Ro-31-8220 + anti-LFA-1

LiCl 1

LiCl 2

LiCl 3

Bisindolymaleimide 1

Okadaic acid 1

Rapamycin 2

Rapamycin 3

Rapamycin 1

Rottlerin 2

H-89 1

Okadaic acid 2

Ro-31-8220 1

-Tubulin

SB203580 3

Go6976 1

Rottlerin 3

Ro-31-8220 + anti-LFA-1

UCN-01 2

UCN-01 3

Staurosporine 1

D

Genistein 2

Genistein 3

Bisindolymaleimide 3

MG132 1

MG132 2

MG132 3

Ro-31-8220 2

Nocodazole 1

Paclitaxel 1

Paclitaxel 2

Paclitaxel 3

F-actin

Brefeldin A 3

Brefeldin A 2

A

Brefeldin A 1

H-89 2

Nocodazole + anti-LFA-1

Cytochalasin D 1

Cytochalasin D 3

Cytochalasin D 2

Calyculin A 2

Ro-31-8220 3

Nocodazole 2

Nocodazole 3

E

AG490 1

AG490 2

AG490 3

-Tubulin

Okadaic acid 3

Calyculin A 1

Poly Lysine

Nocodazole + anti-LFA-1

Figure 4


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