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INFLUENZA TRANSPORT MEDIUM

INFLUENZA TRANSPORT MEDIUM. MEDIA PREPARATION TEAM Miguel Garza, Supervisor Martha Thompson, Viral Isolation Laboratory. Ingredients. Tryptose Phosphate Broth Gelatin Penicillin 250,000 units per mL Streptomycin Sulfate 0.25 grams per mL Distilled Water. Preparation.

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INFLUENZA TRANSPORT MEDIUM

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  1. INFLUENZA TRANSPORT MEDIUM MEDIA PREPARATION TEAM Miguel Garza, Supervisor Martha Thompson, Viral Isolation Laboratory

  2. Ingredients • Tryptose Phosphate Broth • Gelatin • Penicillin 250,000 units per mL • Streptomycin Sulfate 0.25 grams per mL • Distilled Water

  3. Preparation • Combine in a 4 liter flask • Dissolve by stirring • Autoclave 30 minutes at 121° C • Add aseptically Penicillin 250,000 units and Streptomycin Sulfate 0.25 grams • Dispense aseptically 3.0 mls to 1998(aprox.) sterile tubes

  4. Label each tube: INFLUENZA TRANSPORT, 93515, lot number, expiration and Texas Dept. of State Health Services, Austin, TX 78756 • Expiration date is 12 months from date of preparation • Store in Media Production Freezer at -25 °C • Deliver 7 tubes to Virology Team for quality control

  5. QC of Transport Media Sterility Toxicity Growth assay Storage at -70° C for 72 hrs Storage at 4° C for 24 hrs Undilute, 1:5, 1:10 Parallel testing old/new lots

  6. Viral Transport Media • Viral transport medium • Hanks balanced salt solution • Tryptose-phosphate broth • Sucrose-phosphate broth • Cell culture medium • Veal infusion broth • Supplement with a protein or stabilizer, bovine serum albumin or gelatin at 0.5 to 1%. • Antibiotics

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