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MCB 151

MCB 151. Fall 2018. Course Information. MCB 151 Website: http://www.life.uiuc.edu/mcb/151/ Login: NetID Password: AD password. Required Materials. Required Texts and Supplies 1. "Laboratory Exercises for MCB 151: Molecular & Cellular Biology, Fall 2018" Melissa Reedy, editor

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MCB 151

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  1. MCB 151 Fall 2018

  2. Course Information • MCB 151 Website: http://www.life.uiuc.edu/mcb/151/ • Login: NetID • Password: AD password

  3. Required Materials • Required Texts and Supplies • 1. "Laboratory Exercises for MCB 151: Molecular & Cellular Biology, Fall 2018" Melissa Reedy, editor • Stipes Publishing •  2. Biology Laboratory Notebook (carbonless) • Stipes Publishing • Recommended (Optional) Texts • Textbook ISBN is: 0134243064. Biological Science (Freeman et. al, 6th edition) - currently used in MCB 150

  4. Add, Drop, Section Change • Students may use the UI–Integrate Self-Service System to add or change MCB 151 laboratory sections before 5:00 PM, Monday, September 10, 2018 • Friday, October 19 is the last day to drop the course or to elect the Credit/No Credit option. Students may use the UI-Integrate Self-Service System to drop MCB 151 until this deadline.

  5. Course Grading(1000 points total) • Pre-Laboratory Exercises • 9 sets @ 7 points each, 50 points (drop 13 points) • Post-Laboratory Exercises* • 10 sets @ 15 points each, 125 points (drop 25 points) • Laboratory Reports • 8 @ 75 points each, 525 points (drop 75 points) • Laboratory Exams • 2 @ 100 points each • Final Exam • 1 @ 100 points • *Only students that are present and on time are eligible to complete the Post-Lab Exercise and Lab Report for that particular week. If you are absent/late, you may complete the Post-Lab Exercise for your own benefit (i.e. preparation for exams), but you will NOT earn points for it. If you are absent/late, you are not eligible to complete the Lab Report for that particular week and you may not be eligible to submit the previous weeks Lab Report.

  6. Reading Assignments The Reading Assignment for each week can be found on the course web site under the Laboratory Exercises link. These readings supplemental, all material will come from the lab manual and lab experience.

  7. http://www.life.illinois.edu/mcb/151/

  8. LON-CAPA • You will have Pre/Post Lab Assignments each week. Please make sure to follow the directions on pgs. I-18 your lab manual.

  9. LON-CAPA

  10. http://police.illinois.edu/emergencyplanning/ • In an emergency in this building, we’ll have three choices: RUN (get out), HIDE (find a safe place to stay inside), or FIGHT (with anything available to increase our odds for survival). • First, take a few minutes this week and learn the different ways to leave this building. If there’s ever a fire alarm or something like that, you’ll know how to get out, and you’ll be able to help others get out too. • Second, if there’s severe weather and leaving isn’t a good option, go to a low level in the middle of the building, away from windows. • If there’s a security threat, such as an active shooter, we’ll RUN out of the building if we can do it safely or we will HIDE by finding a safe place where the threat cannot see us. We will lock or barricade the door and we will be as quiet as possible, which includes placing our cell phones on silent. We will not leave our area of safety until we receive an Illini-Alert that advises us it is safe to do so. If we cannot run out of the building safely or we cannot find a place to hide, we must be prepared to fight with anything we have available in order to survive. Remember, RUN away or HIDE if you can, FIGHT if you have no other option. • Finally, if you sign up for emergency text messages at emergency.illinois.edu, you’ll receive information from the police and administration during these types of situations. • If you have any questions, go to police.illinois.edu, or call 217-333-1216.

  11. Emergencies in the lab • Eye Wash • Fire Extinguishers • Fire Alarms • First Aid

  12. Course Coordinator Melissa Reedy Course Coordinator MCB 150/151 Office: 415 Burrill Hall Phone: 265-6379 Email: murray@illinois.edu Office hours: By appointment

  13. Exercise 1 Introduction: Lab Techniques and Safety Basic Skills

  14. Laboratory Safety • Bacteria: • Wear gloves • Wash you hands before leaving the lab • Notify your TA of any spills or contamination • Chemicals: • Always handle reagents with caution • UV Light: • Never look at UV lamps • Wear protective gloves

  15. Laboratory Techniques • Clean up: each student is responsible for cleaning up after every experiment • Labels: always label your experimental work with your name and section, b/c we share incubators, refrigerators, water baths, etc. • Pipetting: always review the proper use of pipettes before beginning experiments • Centrifuges: used to spin down and separate

  16. Lab Safety Importance • Work you are doing now can have consequences now and in the future • Toxic or infectious substances could result in acute or chronic illness or even death. • Explosions or fires can injure or kill. • Knowledge of safety is critical in protecting yourself and others from potential hazards.

  17. Housekeeping • Bench tops, floors and other surfaces must be kept clean and free of clutter • Coats and backpacks must be hung on coat hooks in designated areas • To avoid accidental ingestion of toxic or infectious materials, the following are prohibited in the lab: • Eating or drinking • Taking medication • Applying lip balm, gloss, lipstick or make-up

  18. Hand Hygiene • Wear gloves in the lab when directed to do so • Make sure to choose gloves that are the appropriate size and fit well • Do not use gloves that have holes or cracks • Always remove gloves before leaving the room • Remove gloves by pulling cuff down over your hand, turning the glove inside out • Never touch door knobs or handles with gloved hands • Always wash hands after removing gloves

  19. Personal Protective Equipment • Students may elect to wear safety glasses or goggles, which are recommended but not required • Students may also elect to wear lab coats • PPE can be purchased from: • Life Sciences storeroom, C107 CLSL, phone: 217-333-7693 • RAL storeroom, 94 RAL, phone: 217-333-3564

  20. Personal Attire in the Lab • The following guidelines apply to attire in the lab: • Clothing should cover legs and midriff • Footwear should completely cover feet (no open-toed shoes or sandals) • Long hair should be tied back • Loose sleeves or tops should be confined

  21. Biosafety Levels • Biosafety is the application of a combination of laboratory practices and procedures, laboratory facilities, and safety equipment when working with biological material • Biosafety levels: • BL1 - Agents not known to cause disease consistently in healthy humans • BL2 - Agents associated with human or animal disease • BL3 - Indigenous/exotic agents associated with human or animal disease and with potential for aerosol transmission • BL4 - Dangerous/exotic agents of life threatening nature

  22. Sharps • The Illinois EPA defines sharps as: • Any medical needle • Syringe, with or without needle • Scalpel and razor blade • Blood vials • Pasteur pipets • Microscope slides and coverslips • Glassware contaminated with infectious agents • Sharps must be disposed of in approved Sharps container

  23. Biological Waste • Cultures, stocks and disposable labware generated from experiments with biological materials must be autoclaved prior to disposal • Place these materials, as directed, into red biological waste disposal bags in the lab • Do NOT put these materials into regular trash • If you are uncertain, please ask!

  24. Emergency Preparedness • Please note the location of the following: • Shower • Eyewash • Fire extinguisher • Spill kit • Nearest exit • If you must, evacuate to prevent or minimize injury

  25. Fire Safety • If fire occurs: • Notify those in the immediate area • Activate nearest fire alarm • Confine the fire by closing doors and windows • Attempt to extinguish the fire only if it is safe to do so • Do not use elevators • If possible, call 911 to explain the nature of the fire or emergency and meet responders outside

  26. DRS • The DRS website provides campus-specific safety information such as: • Safety guidance documents in the "Safety Library” • Online Safety Trainings • Information on how to manage hazardous waste • Information on DRS safety programs • Accident response information • Templates and forms for managing hazards in the laboratory

  27. Exercise 1B:Basic Laboratory Skills ,

  28. Goals • Understand and manipulate exponential numbers. • Become familiar with the units used for small concentrations and volumes and be able to make conversions between them. • Figure out concentrations of solutions or cell suspensions after dilution. • Understand what is meant by pH and know how to calculate the pH of a solution. • Understand how buffers work.

  29. Exponential Numbers • Every number can be expressed as the product of two numbers, the second one being some power of ten. • For example, the number 100 can be expressed as 10 X 10 = 102 • The same rules are used to express numbers less than one. 10-2 = 1/10 X 1/10 = 1/100

  30. Units of Measurement Common units Mass 1 gram (g) = 103 milligrams (mg) = 106 micrograms (µg) = 109 nanograms (ng) Volume 1 liter (l) = 103 milliliters (ml) = 106 microliters (µl) 1 ml = 1 cubic centimeter Length 1 meter (m) = 103 millimeters (mm) = 106 micrometers (µm) = 109 nanometers (nm)

  31. Dilutions C1V1 = C2V2 Example:Given a 1 M stock solution of Tris buffer prepare 10 ml of 0.01 M Tris. Solution:The question you need to answer is "what volume of 1 M Tris do you need to dilute to give a total volume of 10 ml of 0.01 M Tris". Thus, you know C1, C2, and V2. (? ml x 1 M) = (10 ml x 0.01 M) ? ml = 10 ml x 0.01 M (1 M) Answer: 0.1 ml

  32. Dilution Factor Dilution = 1/100 Dilution Factor: 100 Dilution = 1/n Dilution Factor: n Serial Dilution: product of the individual dilutions 1/102 x 1/10 x 1/103 = 1/106 = 10-6

  33. Aqueous Solutions An aqueous solution is one in which water is the solvent. Water properties: • High surface tension • High boiling point • Good Solvent

  34. Acids, Bases, pH and Buffer Acids/Bases: Bronsted Definition: an acid is a molecule that donates protons and a base is a molecule that accepts protons pH: Dependent upon the concentrations of H+ and OH- ions Buffers: A molecule that allows the solutions to resist changes in the hydrogen ion concentration

  35. Assignments for next week • Post Lab 1 and Pre Lab 2 are assigned today and due before your lab meets again in LON-CAPA. • You must come to lab next week with your pre lab write up complete for Exercise 2B Parts 1 and 2 • Purpose • Procedure/Protocol

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