1 / 1

NT x 10 3

A. WRL-68. Huh6. CHANG. *. *. *. *. *. HepG2. Huh7. HLE. NT x 10 3. *. *. *. *. *. *. NT x 10 3. B. 0-Time 24 h 48 h. Chang + sD. HepG2 + sD. HLE + sD. HepG2. Chang. HLE. S. S. S. C. C. C. sD. sD. sD. DNA-PKcs. β -Actin.

wattan
Download Presentation

NT x 10 3

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. A WRL-68 Huh6 CHANG * * * * * HepG2 Huh7 HLE NT x 103 * * * * * * NT x 103 B 0-Time 24 h 48 h Chang + sD HepG2 + sD HLE + sD HepG2 Chang HLE S S S C C C sD sD sD DNA-PKcs β-Actin Supplementary Figure 1. Inhibition of DNA-PKcs expression by specific siRNA against-DNA-PKcs (sD). (A) DNA-PKcs mRNA levels, in the presence of 1000 ng,of scrambled siRNA (S) or sD, were determined by quantitative RT-PCR real-time. N Target (NT) = 2-ΔCt; ΔCt = RNR18-Ct target gene. Data are means ± SD of 9 experiments. S vs untreated control (C) and sD vs S, P < 0.0001 at 48h for all cell lines. (B) Representative western blot analysis showing efficient DNA-PKcs silencing by specific siRNA (sD) in Chang, HepG2, and HLE cell lines when compared to control (untreated counterparts). Protein values 48 h after sP are shown.

More Related