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Investor Presentation. Pressure BioSciences, Inc. ( OTCQB : PBIO) Discovery Starts with Sample Preparation™ 3 rd Global Life Sciences Conference Warsaw Stock Exchange -Trading Floor Warsaw, Poland June 11, 2015. Forward Looking Statements.

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Investor Presentation

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  1. Investor Presentation Pressure BioSciences, Inc.(OTCQB: PBIO)Discovery Starts with Sample Preparation™ 3rd Global Life Sciences Conference Warsaw Stock Exchange -Trading FloorWarsaw, PolandJune 11, 2015

  2. Forward Looking Statements This presentation may contain forward looking statements that reflect management’s current views and opinions as to the status of the Company’s products, technology and other future events and operations. These statements are neither a promise nor guarantee, but involve risks and uncertainties that could cause actual results to differ materially from those anticipated or indicated. Investors are cautioned that any forward looking statements should be considered in light of such risks and uncertainties including, without limitation, those detailed in the Company’s filings with the Securities and Exchange Commission. 2

  3. 10 FT Staff; Near Boston, MA; Publicly Traded (OTCQB: PBIO) 24 Patents: Pressure Cycling Technology (“PCT”) Platform Develop, Mfg, & Sell PCT-based Instruments & Consumables Initial Focus in Sample Preparation for the Research Market 250 PCT Systems Installed, 150 Customers, 100+ Publications Early Product Revenue: 2014 ($1,349,524) New Instruments (HUB880 and Barozyme HT48) and Consumable (micro-Pestle) Releases in 2014 Expected to Significantly Impact Growth in 2015 and Beyond We Plan to Substantially Add to our Sales and Marketing Capabilities in 2015 3 Company Overview (OTCQB: PBIO) 3

  4. Management Mr. Richard T. Schumacher, President & CEO Boston Biomedica (CEO, Founder); Panacos Pharma (President, Co-founder); Trinity Biotech (Founding Group); CBR Labs (Gen’l Mgr) - Harvard Medical School Dr. Edmund Y. Ting, Senior Vice President of Engineering Avure Technologies (CSO); Flow Int’l (CSO); Grumman Aerospace; MIT (Ph.D.) Dr. Alexander V. Lazarev, Vice President of R&D Proteome Systems; Genomic Solutions; ESA; University of Kazan (Ph.D.) Dr. Nathan P. Lawrence, Vice President of Marketing Boston Biomedica; BD; Gene-Trak Systems; Yale University (Ph.D.) Mr. Richard P. Thomley, Chief Financial Officer Kiva Systems; SynQor; Catamount Manufacturing; ChemDesign Corp Board Jeffrey Peterson, MS Chairman Kevin Pollack, Esq., MBA Audit Committee Chairman Vito Mangiardi, MBA Compensation Committee Chairman Mickey Urdea, Ph.D. SAB Chairman Richard T. Schumacher CEO, Treasurer, Clerk 4 Experienced Senior Management & Board

  5. Primary Sample Preparation for Biomolecular Analysis: Cell Lysis Pressure Cycling Technology (PCT) 5

  6. Scientific research can be broken down to three key elements: sample preparation, analysis, & data reduction/interpretation “Discovery Starts with Sample Preparation” Sample Input Quality = Sample Result Quality 6

  7. Analysis of DNA, RNA, Proteins, & Lipids (“biomolecules”) is Crucial to the Discovery of New Biomarkers – Quality Depends on Quality of Preparation Biological Sample Preparation is a Multi-Billion Market Comprised of an Estimated 500,000 Scientists in 80,000 Research Labs WW Current Sample Prep Methods are Highly Inadequate: Bottleneck Proven Platform: ~ 250 PCT Systems Installed (~150 Sites) 100+ Publications Highlight Advantages of PCT Platform Over Other Methods 2014: Completed Development of HUB880 Instrument, the Barozyme HT48 High-throughput Instrument System, and the µPestle Consumable. These Products Expected to Impact Growth in 2015 and Beyond. 2015: Company Receives PO for First Barozyme HT48 System…Company Awarded $1M NIH Grant (DNA Shearing)…Awarded $1M NIH Grant…PCT-HD Released (µPestle) for Novel Preparation of Small Biopsy Tissues…Professor Aebersold & Team Publish on PCT-HD (PCT SWATH) in Nature Medicine 7 Value Proposition: PCT-based biological sample preparation 7

  8. Estimated Market Genomics (DNA/RNA) $7.1B in 2015 b Next Gen Sequencing (DNA) $2.7B in 2017c Mass Spectrometry (Proteins) $2.7B in 2011a,d Forensic Tools and Products (DNA) $17.7B in 2019 e Sample Prep for all OMICS: $8.4B in 2016 f 500,000 Scientists in 80,000 Biological Research Labs WW a a EMMES GroupbDecisive Bio-Insights c Markets & Markets dTechNavioe Transparency Market Rshf bcc Research Biomarker/Forensics Market: Estimated Size 8

  9. Selected Marquee Customers (150) 9

  10. Competitive Landscape Nearly All Competitive Methods Involve Mechanical Disruption • Mortar & Pestle • Dounce homogenizer (glass on glass) • Potter-Elvenhjem homogenizer (Teflon on glass) • Enzymatic Digestion • Polytron shearing homogenizers • Blenders • Bead Beating • Sonication • Repeated Freeze/Thaw cycles • French Press (≤ 2000 PSI) 10

  11. PCT Sample Preparation System Barocycler™ NEP2320 Barocycler™ NEP3229 PULSE Tube PCT-dependent Kits MicroTubes 11

  12. The Solution (4 years in the making) Barozyme HT48 Initial Evaluation (KOLs) Nov/Dec 2015 1st Sale February 2015 • Initial Application: • High pressure digestion • Consumable: • Proprietary 8-well strips • Number of Samples: • 48 samples 12

  13. PCT Platform: Enabling the Enablers Eight Impact Areas Where PBI’s Powerful and Enabling PCT Platform is Currently Being Used… and the Early Results are Very Exciting 1. Agriculture/Food Science 2. Environmental Epidemiology 3. Anti-Bioterror 4. Rational Drug Design 5. Biomarker Discovery 6. Fecal Lipidomic Profiling 7. Personalized Medicine 8. Improved Rape Kit Testing 13

  14. Agriculture and Food Science • Dr. Patricia Okubara • Research Geneticist (Plants) • U.S. Department of Agriculture (USDA-ARS) • Adjunct Professor, WSU • Use of pressure cycling technology for cell lysis • and recovery of bacterial and fungal communities • from soil. BioTechniques, Vol. 58, No. 4, April 2015. • Two standard cell lysis methods were compared to PCT for direct extraction of DNA in soil • PCT extracted DNA from a greater number of unique bacterial and fungal species (greater diversity) • Conclusion • The determination of microorganism diversity in soil is critical for successful crop growth and pestilence control. PCT extraction resulted in greater diversity of microorganisms; thus, PCT may be a valuable alternative to current lysis methods 14

  15. Environmental Epidemiology • Dr. William E. Funk • Assistant Professor in Preventive Medicine-Cancer • Epidemiology and Prevention Northwestern University, • Feinberg School of Medicine • Quantification of cotinine in plasma and dried blood • spots using nano-chip LC-MS. 2015 NU Research Day • PCT extraction of cotinine (predominant metabolite of • nicotine) from dried blood spots faster with excellent yields • PCT HT48 System amenable to the high-throughput studies required • for population-based environmental studies • Conclusion • PCT effectively extracted cotinine from dried blood spots; • PCT may one day be used to extract and help measure • other potential chemical toxins and carcinogens from environmental sources other than tobacco • 15

  16. Anti-bioterrorism • Dr. Bradford Powell • Director Analytical Sciences, PharmAthene • Previously Division of Bacteriology, US Army Medical • Research Institute of Infectious Diseases (USAMRIID) • Inactivation and Extraction of Bacterial Spores • for Systems Biological Analysis • Sample Preparation in Biological Mass Spectrometry • PCT is useful for preparing multiple classes of analytes, including nucleic acids, carbohydrates and lipids  • PCT can be exploited for differential extraction and preparation of sub-spore components and for recovery of spores from infected host tissue • Conclusion • Ultra-high pressure cycling can achieve complete inactivation of Anthrax with extraction in a single tube, useful for whole analysis • of the spore proteome • 16

  17. Rational Drug Design and Development • Professor Wayne L. Hubbell • Distinguished Professor of Biochemistry: UCLA • Member, National Academy of Sciences • Fellow, American Academy of Arts and Sciences • Structure-Relaxation Mechanism for the • Response of T4 Lysozyme Cavity Mutants to • Hydrostatic Pressure. PNAS: April 2015 • On-going need to improve drug discovery, design & development • Molecular structure of a protein and the conformational changes that control • its function are key to better drug design and development • Conclusion • Response to Pressure May Have Profound Implications in • Future Drug Design & Development • 17

  18. Biomarker Discovery • Professor Jennifer Van Eyk • Professor of Medicine, Cedars-Sinai Med. Ctr. • Director, Basic Science Research, • the Barbara Streisand Women’s HC • Improved protein extraction and identification • from archival formalin-fixed paraffin-embedded • human aortas. Proteomics Clin. Appl. April 2013 • Over 1 billion archived formalin-fixed paraffin-embedded (FFPE) samples exist, representing an unparalleled set of samples for clinical investigation • Combination of heat and elevated pressure increased protein yield from FFPE aorta samples, resulting in significantly more proteins identified • Conclusion • Results suggests that archival and often rare FFPE aortas and other tissues from patients with clinical histories that often span decades could potentially be invaluable if made available for PCT-enhanced • biomarker discovery and quantification 18

  19. Non-invasive Fecal Lipidomic Profiling • Professor Bruce Kristal • Neuroscientist, Brigham and Women's Hospital, Boston • Associate Professor of Neurosurgery, Harvard MS • Molecular Biologist, Expert in Metabolomics • Method Development for Fecal Lipidomics ProfilingAnal. Chem. 2012 • Robust methodologies for the analysis of fecal material will facilitate the understanding of gut (patho)physiology and its role in health and disease • and will help improve care for individual patients, especially infants • Pressure cycling differentially increased the yield of a number of lipids • Conclusions • Non-invasive fecal lipidomic profiling to define gut function • in health and disease could help physicians assess • intestinal function, determine gut response to nutrition, • and recognize existing inflammation/pathology… • especially valuable in infants and the elderly 19

  20. Improved Rape Kit Testing • Professor Bruce McCord • Professor of Analytical/Forensic Chemistry, FIU • Over 75 peer reviewed papers and 11 book chapters • The application of alkaline lysis and PCT in the • differential extraction of DNA from sperm and • epithelial cells recovered from cotton swabs • Analytical and Bioanalytical Chemistry, In Press • An estimated 400,000 untested rape kits in the USA • (The Dark Side of Clearing America’s Rape Kit Back-log, Time Magazine 2013) • This current study presents a novel technique to extract biological fluids from • cotton swabs with high recovery using pressure cycling technology • Conclusion • Short extraction times, high yields, inexpensive reagents • and semi-automated platform make alkaline lysis-based PCT • a promising candidate for extracting DNA from rape kits with the • potential additional benefit of reducing the current backlog 20

  21. Personalized Medicine: Analysis of Tissue Biopsies • Professor Ruedi Aebersold • Professor of Systems Biology, Institute of • Molecular Systems Biology (IMSB), ETH Zurich • Scientific Advisory Committee of numerous public and • private research organizations; member of several • editorial boards in proteomics & genomics • Rapid mass spectrometric conversion of tissue biopsy • samples into permanent quantitative digital • proteome maps. Nature Medicine March 2015 • New PCT-based method ("PCT-SWATH“) described for proteomic analyses of • tissue biopsies with unprecedented speed and precision. • Thousands of proteins can be quantified vs. hundreds by standard method. • Conclusion • Authors expect wide applications of PCT-SWATH • in personalized medicine, and believe that PCT-SWATH may • ultimately achieve clinical adoption • 21

  22. Mr. David Weild IV • Founder, Chairman, and CEO, IssuWorks • CEO, Weild & Co Holdings (Inv Banking Firm) • Former Vice-Chairman of NASDAQ • Former Head of Global Equity Transactions, • Prudential Securities • Former President, PrudentialSecurities.com • Former Head, Capital Markets Group of Grant Thornton LLP • Known as the “Father of the JOBS Act”, according to Forbes Magazine • Pressure BioSciences Engages IssuWorks in Strategic Advisory Role • Pressure BioSciences has retained IssuWorks and their management • team to review strategic and financing alternatives 22

  23. Key Investment Highlights • Pressure Cycling Technology (PCT): Powerful, Enabling, Patented • Proven Technology: ~ 250 Installations in ~150 Marquee Laboratories • Experienced Management Team and Board of Directors • Large Market Opportunity (est. $6 Billion; 80k Labs) • Short Term Growth Drivers (2015) • On-going Discussions with Large Potential Strategic Partners • “Razor/Razorblade”Business Model (Product Rev Up 30% FY2014) • Possible Spin-off of Vertical Market Applications • New $1 Million NIH Grant (helps cover OH; allows entrance to DNA Field) • 2014 Product Releases Expected to Significantly Impact 2015 Revenue • HUB880u-Pestle (PCT-HD)Barozyme HT48 23

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