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The unique system having an internal cut-off control

The unique system having an internal cut-off control. ••• Procedure. ••• Interpretation. ••• Interpretation. The positive control DOT must be positive (coloured) in all cases The negative (cut-off) control DOT gives the extent of non-specific antibody binding of the sample in the test.

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The unique system having an internal cut-off control

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  1. The unique system having an internal cut-off control

  2. •••Procedure

  3. ••• Interpretation

  4. ••• Interpretation • The positive control DOT must be positive (coloured) in all cases • The negative (cut-off) control DOT gives the extent of non-specific antibody binding of the sample in the test. • The central DOTS, coated with the specific antigens, give the results when compared to the Negative DOT. The colour intensity depends of the titer of the specific antibody in the tested sample. • To be positive, the intensity of the colour must be higher than the Negative DOT. • When the intensity of the colour is below the Negative DOT, the result is negative. • When the intensity of the colour is equal to the intensity of the Negative DOT, the result is considered negative.

  5. •••A full DOTS strategy for systemic diseases The detection of HEp-2 positive patients requires further identification steps of the autoimmune markers. Our full DOTS programme, entirely automatisable, allows the identification of all the autoantibodies correlating with systemic autoimmune diseases... OUR DOTS PROGRAMME SYSTEMIC AUTOIMMUNE DISEASES ••• Systemic Lupus Erythematosus (SLE) ••• Mixed Connective Tissue Disease (MCTD) ••• CREST Syndrome ••• Progressive Systemic Sclerosis (PSS) ••• Rheumatoid Arthritis (RA) ••• and related overlap... ••• and polyautoimmune syndromes...

  6. ••• ENA(Sm, Sm/RNP, SSA, SSB, Jo1, Scl70)

  7. ••• ENA(Sm, Sm/RNP, SSA, SSB, Jo1, Scl70) •••Nucleosomes + Histones •••Lupus Screen(Nucleosomes, Histones, Sm, Ribosomes)

  8. •••Nuclear and Cytoplasmic Antigens

  9. •••Nuclear and Cytoplasmic Antigens

  10. •••Nuclear and Cytoplasmic Antigens

  11. •••Cytoplasmic AntigensHEp2 cytoplasmic patterns have to be characterized and differentiated

  12. •••Profiling the liverautoimmune diseases with our full liver dots programme OUR FULL DOTS PROGRAMME : You have to cover a large panel of liver autoimmune pathologies characterized by serological markers...but detection and differentiation are frequently uneasy... AUTOIMMUNE LIVER DISEASES ••• Primary Biliary Cirrhosis (PBC) ••• Autoimmune Hepatitis type I (AIH I) ••• Autoimmune Hepatitis type II (AIH II) ••• Post infectious autoimmune hepatitis... HOW HELPING DIAGNOSIS WITH SEROLOGY ? ••• F-Actin Specific AIH type I marker ••• LKM1 Specific AIH type II marker ••• LC1 An AIH type II sometimes present in LKM1 negative patients ••• SLA Soluble Liver Antigen remains one of the rare but classical markers of AIH ••• M2 PDH Specific PBC marker

  13. •••Liver markers

  14. •••Primary Biliary CirrhosisAMA •••5% of the PBC are PDH negative •••The E2 sub-units of the 3 complexes Are the specific PBC EPITOPES

  15. •••The Actin confusion SMA..........non F-Actin..........F-Actin remain a « mix-up » ••• Only anti-F(filamentous) Actin antibodies are the specific markers of AIM type I. ••• Anti-SMA (desmin, reticulin, vimentin, actin...) do not correlate with AIH type I.

  16. ••• Profiling your patientswith autoimmune systemic vasculitis THE INFAILLIBLE STRATEGY : Vasculitis is a complex family of diseases... DETECTING and DIFFERENTIATING among the different vascular pathologies those with specific markers is a PRIORITY allowing an urgent and efficient THERAPY... AUTOIMMUNE SYSTEMIC VASCULITIS : • Wegener disease • Rapid Progressive Glomerulonephritis • Goodpasture syndrome OUR AUTOIMMUNE SYSTEMIC VASCULITIS STRATEGY GIVES YOU IN ONE RUN A CORRECT CLASSIFICATION USING THE MOST RELIABLE IFA, DOTS AND ELISA KITS...

  17. •••Vasculitis markers

  18. •••We are also concerned with less prevalent autoimmune diseases WE SUGGEST YOU : The DOT method is particularly adapted to the detection of rare antibodies remaining convenient and economical ARE CONCERNED : ••• Biermer’s pernicious anaemia ••• Atrophic gastritis ••• Stiff Man neurologic syndrome

  19. •••Biermer’s anaemia markers

  20. •••Neurologic markers

  21. ••• Food intolerance is today a majorconcern… frequently mistaken for allergy ! INTOLERANCE PATHOLOGIES CONCERN MAINLY : ••• Gluten ••• Cowmilk proteins ••• Soya HOW CAN SEROLOGY DIFFERENTIATE FOOD INTOLERANCE FROM ALLERGY ? •••ALLERGY involves IgE antibodies •••INTOLERANCE involves IgA and IgG antibodies

  22. •••Cœliac disease markers

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