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pcDNA

a. IP. WB: a -PABP. Input. Sam68 D C. pcDNA. D 28 D C. D Cmin. 5-262. }. }. }. }. }. Sam68 D C. D 28 D C. pcDNA. D Cmin. 5-262. PABP. R-IgG. R-IgG. R-IgG. R-IgG. R-IgG. PABP. PABP. PABP. PABP. Ab:. 95. 72. 72. b. IP. Sam68 D C. pcDNA. D 28 D C. D Cmin. Input.

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pcDNA

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  1. a. IP WB: a-PABP Input Sam68DC pcDNA D28DC DCmin 5-262 } } } } } Sam68DC D28DC pcDNA DCmin 5-262 PABP R-IgG R-IgG R-IgG R-IgG R-IgG PABP PABP PABP PABP Ab: 95 72 72 b. IP Sam68DC pcDNA D28DC DCmin Input 5-262 } } } } } Sam68DC R-IgG PABP R-IgG PABP R-IgG PABP R-IgG PABP R-IgG PABP pcDNA D28DC DCmin 5-262 9 kb 2 & 4 kb actin c. 2.5 ** 2.0 1.5 PABP Associated 9kb RNA 1.0 ** 0.5 0.0 5-262 D28DC pcDNA DCmin Sam68DC

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