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Lab 3 Connective tissue (2)

Lab 3 Connective tissue (2). Cartilage,Bone and Blood smear. Jun Zhou( 周俊） , Ph.D & M.D School of Medicine,Zhejiang University. Slides. 1.cartilage ( No.3 trachea) 2.Bone (No.5) 3.Blood smear (picture drawing). Hyaline Cartilage. C-shape hyaline cartilage. Trachea (No.3).

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Lab 3 Connective tissue (2)

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  1. Lab 3 Connective tissue (2) Cartilage,Bone and Blood smear Jun Zhou(周俊）, Ph.D & M.D School of Medicine,Zhejiang University

  2. Slides • 1.cartilage ( No.3 trachea) • 2.Bone (No.5) • 3.Blood smear (picture drawing)

  3. Hyaline Cartilage C-shape hyaline cartilage Trachea (No.3)

  4. Hyaline Cartilage Trachea(No.3)：H&E ×40

  5. capsule lacuna perichondrium Isogenous groups Trachea：Hyaline Cartilage H&E ×400

  6. Bone • Haversian system(osteon) • Central canal • Circumferential lamella • Interstitial lamella • Bone lacuna • Bone canaliculus Silver ×100

  7. Haversian system(osteon) • Central canal • Bone lacuna • Bone canaliculus Bone：Silver ×400

  8. perforating (Volkmann’s) cannals Bone：Silver ×100

  9. Blood smear: A drop of blood is placed directly on a glass slide and spread over its surface with the edge of another slide. Stained with Wright’s stain.

  10. Erythrocytes: • 7-8um • Biconcave disc • Stain uniformly with eosin • Neutrophil: • proportion - 50-70%; • Most numerous • Multilobed nucleus • Pale pink granules Blood smear：Wright ×400

  11. Eosinophils. • 2-4% • 8-10 microns morphology • bilobed nucleus large, • uniform eosinophil granules. Blood smear：Wright ×400

  12. Basophils. • Proportion 0.5% • morphology - large, irregular basophil granules obscure the nucleus Blood smear：Basophil Giemsa stain ×400

  13. Lymphocyte: • Intensely stained nucleus • Possess a slight indentation • Small amount of cytoplasm • Pale blue Blood smear：Wright ×400

  14. Monocyte: • nucleus ovoid to kidney shaped • blue-gray Cytoplasm Blood smear：Wright ×400

  15. Platelets: • Small speck-like objectives • Aggregated into small groups • From megakaryocytes in myeloid tissue. Blood smear：Wright ×400

  16. Blood smear：Wright ×400

  17. THE END!

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