Tuning the F unctional and Mechanical P roperties of Multiphoton Fabricated Hydrogels

1. Tuning the Functional and Mechanical Properties of Multiphoton Fabricated Hydrogels 20 µm Eric Spivey 24 May 2011

2. Biopolymer Solution Focused, pulsed laser Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix Microscope Objective Motivation: Use this method to fabricate cell scaffolds for neural tissue engineering applications. Goal:Fabricate hydrogel microstructures with tunable functional and mechanical properties.

3. Dynamic Mask Multiphoton Lithography

4. Cellular Microenvironments 10 µm 25 µm

5. Tuning Young’s Modulus 5 µm 20 µm 10 µm

6. Tuning Young’s Modulus BSA and GMHA Gels PEG and GMHA Gels

7. Functionalizing Hydrogels 1 mW 2 mW 3 mW 4 mW 20 µm

8. Ongoing Research 20 µm

9. Questions?