Xanthan Gum

1. Biopolymers Xanthan Gum Mrs.N.Gunasheela Asst Professor,Dept of Microbiology, Sri Ramakrishna College of Arts & Science for Women, Coimbatore.

2. Definition • Any larger polymeric compound(Protein, nucleic acid,polysaccharide,lipids)produced by living organism derived from microbes ,plants and animals.

3. Types of Biopolymers • Xanthan Gum • Melanin • Adhesives • Polyhydroxy Butyrate Aim: Design new biopolymers 1.Replace the synthetic polymers 2.Increased yield and decreased cost

4. Plan & Discussion • Aim: For commercial production of xanthan gum X.campestrisgrown in inexpensive and plentiful carbon source • Wild type X.campestriscan efficiently utilize glucose, sucrose and starch but not lactose as carbon source • WHEY • Waste by-product of cheese making process consists of water,lactose,protein,minerals and low molecular weight organic compounds • In Dairy Industry disposal of whey is a major problem • Release whey in rivers and lakes can deplete the amount of oxygen and kill organisms • Transport landfill is expensive and contaminate the groundwater

5. Synthesis of Xanthan Gum • Xanthomonas campestris-Gram negative obligatory aerobic soil bacterium • Properties: • High molecular weight exopolysaccharide • High viscosity ,stable in extreme physical and chemical properties

6. Aim: Growing genetically engineered X.campestrison whey Methodology

7. Engineering E. coli lacZ (encoding b-galactosidase) and lacY(encoding lactose permease) genes for constitutive expression in X. campestris (Xc) lac z pXc pLac lac y X.Cam gene lacy pXc lac z

8. Tripartite Mating • A process in which conjugation is used to transfer a plasmid vector to a target cell when the plasmid vector is not self mobilizable • The target cells with the plasmid vector are separated from the other cell types in the mixture by various selection procedures.

9. Application