Gel Permeation Chromatography - pdf

1. Gel Permeation Chromatography: is an effective analytical method used to separate highly sensitive molecules by size, depending on their concentration in a column made of a gel-like medium In Gel Permeation Chromatography process, the sample is poured into the column, which may include a semi-permeable membrane or a selective column designed to adsorb certain size classes of substances. The column is primed before use and maintained at specific concentrations for a pre-set period of time. The sample is recovered after retention time and analyzed on a GE Healthcare spectrophotometer. Many industries employ this technique as a way of obtaining reliable data on the composition of key chemicals and other materials that cannot be detected by more conventional techniques. This is because this technique separates highly sensitive substances from the mass background of the chromatogram. Both qualitative and quantitative analyses can be obtained from this method. Gel permeation chromatogram (GPC), a compound of chromatography, is one of the two general-based GPC methods, which relies on the use of a column to trap sample particles on the surface of the column. Read More@ https://bit.ly/2SnmyA8