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microbial manganese oxidizers and reducers in the columbia river

2. Significance:. EstuariesTransition ZoneNutrient RichProtected from OceanHigh BiodiversityMicrobial Populations60-90% Earth's BiomassGeochemical CyclingCarbon FixationManganese UtilizersLargest Mn Source to CA CurrentCarbon FixationDOM from POM. Massive algal bloom off the coast of Vancouver Island BC..

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microbial manganese oxidizers and reducers in the columbia river

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    1. Microbial Manganese Oxidizers and Reducers in the Columbia River By Kira Kranzler Frontline Mentor: Suzanna Brauer Senior Mentor: Brad Tebo

    2. 2

    3. 3 Bacterial Mn Cycle: Links to C cycle

    4. 4 The Calvin Cycle(Carbon Fixation)

    5. 5 The Problem: Showing a concrete link between the RuBisCO gene and Manganese oxidation. RuBisCO gene not shown to function in SI85-9A1. SI85-9A1’s RuBisCO gene HAS been shown to work in E. coli. -Caspi, R., Haygood, M. G. & Tebo, B. M. (1996). Unusual ribulose-1,5-bisphosphate carboxylase/oxygenase genes from a marine manganese-oxidizing bacterium. Microbiology-Uk 142, 2549-2559.

    6. 6 To better understand the Mn utilizing population in the Lower Columbia River Estuary. Grow and culture individual species of Mn utilizing microbes. Determine size of culturable Mn oxidizing and reducing populations in the estuary. Determine potential for C fixation by the Mn oxidizing population, using RuBisCO gene. Objectives:

    7. 7 Sample Collection on the Lower Columbia River Estuary:

    8. 8

    9. 9 The Estuary Turbidity Maximum As a salt wedge from the ocean tide moves upriver, water near the front of the wedge can well upwards and sink back down, scouring the bottom and forcing nutrients and microbes from the river bottom up into the higher water levels.

    10. 10 Microbial growth from the June 14th Columbia River Cruise.

    11. 11 Mn (III)-PPi + Glycerol Plates Show Positive for Manganese Oxidizers

    12. 12 Colony Growth Results from Columbia River Water Samples Plated on Varying C sources

    13. 13 The oxidation of Mn(II) to Mn(IV) passes through a transient Mn(III) intermediate: Mn(II) Mn(III) MnO2 -Trouwborst, R. E., Clement, B. G., Tebo, B. M., Glazer, B. T. & Luther, G. W. (2006). Soluble Mn(III) in suboxic zones. Science 313, 1955-1957. -Webb, S. M., Dick, G. J., Bargar, J. R. & Tebo, B. M. (2005). Evidence for the presence of Mn(III) intermediates in the bacterial oxidation of Mn(II). Proceedings of the National Academy of Sciences of the United States of America 102, 5558-5563.

    14. 14 Manganese Reduction

    15. 15

    16. 16 96 Well MPN Plates

    17. 17

    18. 18 Amplification of RuBisCO gene from Lower Columbia River Estuary water sample

    19. 19 Conclusion: Mn levels in samples parallel ETM events. Mn oxidizers make up a significant percentage of the Lower Columbia River Estuary culturable microbial population that uses succinate or glycerol. Amount of DNA extracted in the ETM event significantly greater than outside the ETM event. Some Mn oxidizers are capable of forming Mn oxides starting from Mn(III).

    20. 20 The Next Steps: Process MPNs from July cruise. Colony PCR and sequencing of isolated Mn oxidizing colonies and compare to community analyses. Clone library of 16S ribosomal gene. Data analysis with BLAST.

    21. 21 Special Thanks To: Suzanna Brauer Brad Tebo Antonio Baptista Andrew Han Craig Anderson Dan Murphy Holly Simon Lydie Herfort Rick Davis Michael Wilkin and the Crew of the Forerunner And everyone else in my lab!

    22. 22 References Caspi, R., Haygood, M. G. & Tebo, B. M. (1996). Unusual ribulose-1,5-bisphosphate carboxylase/oxygenase genes from a marine manganese-oxidizing bacterium. Microbiology-Uk 142, 2549-2559. Pichard, S. L., Campbell, L. & Paul, J. H. (1997). Diversity of the ribulose bisphosphate carboxylase/oxygenase form I gene (rbcL) in natural phytoplankton communities. Applied and Environmental Microbiology 63, 3600-3606. Selesi, D., Schmid, M. & Hartmann, A. (2005). Diversity of green-like and red-like ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes (cbbL) in differently managed agricultural soils. Applied and Environmental Microbiology 71, 175-184. Tebo, B. M., Johnson, H. A., McCarthy, J. K. & Templeton, A. S. (2005). Geomicrobiology of manganese(II) oxidation. Trends in Microbiology 13, 421-428. Tebo, B. M., Bargar, J. R., Clement, B. G., Dick, G. J., Murray, K. J., Parker, D., Verity, R. & Webb, S. M. (2004). Biogenic manganese oxides: Properties and mechanisms of formation. Annual Review of Earth and Planetary Sciences 32, 287-328. Trouwborst, R. E., Clement, B. G., Tebo, B. M., Glazer, B. T. & Luther, G. W. (2006). Soluble Mn(III) in suboxic zones. Science 313, 1955-1957. Webb, S. M., Dick, G. J., Bargar, J. R. & Tebo, B. M. (2005). Evidence for the presence of Mn(III) intermediates in the bacterial oxidation of Mn(II). Proceedings of the National Academy of Sciences of the United States of America 102, 5558-5563 <http://www.iinet.com/~englishriver/LewisClarkColumbiaRiver/Images/columbia_river_estuary_cathlamet_bay_2003.jpg> <http://www.waterencyclopedia.com/images/wsci_01_img0017.jpg> <http://media.portland.indymedia.org/images/2004/07/292339.jpg> <http://www.hpl.umces.edu/~jpierson/images/etmlogo.JPG>

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