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Fluorophores are a family of chemical compounds that can emit light upon the adsorption of energy from an excitation light source. For a certain fluorophore, there is a specific excitation and emission spectrum. Typically, the wavelengths of light that can cause the maximum excitation and be captured from the maximum emission (Excitation/Emission) are used to refer to a given fluorophore.
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Antibody-Fluorophore Probes https://www.creative-biolabs.com/adc
Antibody-Fluorophore Probes • Fluorophores are a family of chemical compounds that can emit light upon the adsorption of energy from an excitation light source. For a certain fluorophore, there is a specific excitation and emission spectrum. Typically, the wavelengths of light that can cause the maximum excitation and be captured from the maximum emission (Excitation/Emission) are used to refer to a given fluorophore.
Antibody-Fluorophore Probes • Most fluorophores are small organic compounds with 20–100 atoms, but there are also exceptions with larger sizes, e.g., natural fluorescent proteins. • Most fluorophores are small organic compounds with 20–100 atoms, but there are also exceptions with larger sizes, e.g., natural fluorescent proteins.
Fluorescent images of human breast cancer MDA-MB-231 cells using fluorescently labelled SH3GL2 antibodies (green, A), anti-mitochondria antibodies (red, B), and PGC-1α antibodies (cyan, C). The merged image showed the co-localization of SH3GL2, PGC-1α, and mitochondria (Clin. Cancer. Res., 2016).
Antibody-Fluorophore Probes • Fluorophores or fluorescent conjugates play important roles in biochemical and biomedical studies and applications. For example, fluorophores can be used as probes for the measurement and tracking of ions, including Ca2+, Mg2+, Zn2+, Hg2+ and Cd2+, as well as for the capture and visualization of small molecules like H2O2, NO and H2S.
Flow cytometry Fluorophore labeled antibodies enable cell sorting techniques such as fluorescence-activated cell sorting (FACS) flow cytometry for the fast and efficient identification and collection of cells expressing the desired surface antigen. In the meantime, with the development of more advanced imaging flow cytometry platforms, antibody-fluorophore probes facilitate not only the sorting and quantification of surface antigen, but also provide the means for the acquisition of high resolution images of the cells bearing the antibody binding targets.
LOREM IPSUM DOLOR With over a decade’s experiences in bio-conjugation chemistry, high-resolution imagery, and with our comprehensive antibody library, Creative Biolabs takes the lead to provide customers with high performance custom antibody-fluorophore probes.
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