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Lecture 20

Lecture 20. Methods to produce transgenic animals. What are Transgenic Organisms?. Transgenic organisms are organisms that are injected with foreign DNA from another organism. http://www.unc.edu/~unclng/cow.jpg. How are they created?. DNA Microinjection Retrovirus-mediated gene transfer

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Lecture 20

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  1. Lecture 20 Methods to produce transgenic animals

  2. What are Transgenic Organisms? • Transgenic organisms are organisms that are injected with foreign DNA from another organism http://www.unc.edu/~unclng/cow.jpg

  3. How are they created? • DNA Microinjection • Retrovirus-mediated gene transfer • Embryonic stem cell-mediated gene transfer

  4. DNA Microinjection • Direct microinjection of a chosen gene construct from another member of the same species or from a different species, into the pronucleus of a fertilized ovum.

  5. Retrovirus-mediated gene transfer • Retroviruses are used as vectors • Infects host cells • Not all cells carry the retrovirus • The retrovirus must integrate into the germ cells to work.

  6. Embryonic stem cell-mediated gene transfer • Insertion of desired DNA sequence into an in vitro culture of embryonic stem (ES) cells. • Stem cells can give rise to a complete organism. • The cells are then incorporated into an embryo at the blastocyst stage of development.

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