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What Should You Know About Dithiothreitol or DTT

DTT or Dithiothreitol is a widely used reducing agent. Upon oxidization, it forms a stable ring with six members through an internal disulfide bond. With a redox potential of -0.33 V at pH 7, it is essential in the production of pharmaceutical raw materials.

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What Should You Know About Dithiothreitol or DTT

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  1. What Should You Know About Dithiothreitol or DTT DTT or Dithiothreitol is a widely used reducing agent. Upon oxidization, it forms a stable ring with six members through an internal disulfide bond. With a redox potential of -0.33 V at pH 7, it is essential in the production of pharmaceutical raw materials. If you require it for the production of medicines, then contact Apis AndIntermediates Suppliers, Manufactures China. The purpose As already mentioned, DTT is a reducing or a deprotecting agent used on thiolated DNA. The terminal sulfur atoms of thiolated DNA generally ends up forming dimers in solution. It happens primarily due to the presence of oxygen. DTT also proves useful for the reduction of proteins and disulfides. It prevents intermolecular and intramolecular disulfide bonds from intruding between cysteine residues of proteins. The properties DTT Manufacturers China informs you that DTT is a relatively unstable aggregate. The problem arises from swift air oxidization. You can extend its valuable life by refrigeration. You should also handle the compound in an inert atmosphere. Oxidation leads to further complications as DTT exhibits a sharp absorption peak at 260 nm. More information The half-life of DTT is about forty hours at 6.5 pH. The same compound loses its integrity within 1.4 hours if the pH rises to 8.5 and the temperature reaches twenty-degree-centigrade. DTT’s half-life decreases even further with increasing temperature.

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