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Sedimentation

Sedimentation. Principle. centrifugal force - buoyancy = frictional force. w: angular velocity r: distance from the center of spinning m: mass of the molecule n: partial specific volume r : density of solution v: velocity of sedimentation f: frictional coefficient.

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Sedimentation

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  1. Sedimentation Biophysical Tools '02 - Sedimentation

  2. Principle centrifugal force - buoyancy = frictional force w: angular velocityr: distance from the center of spinningm: mass of the moleculen: partial specific volumer: density of solutionv: velocity of sedimentationf: frictional coefficient Sedimentation coeff. = velocity per unit centrifugal field Biophysical Tools '02 - Sedimentation

  3. Analytical Centrifuge Biophysical Tools '02 - Sedimentation

  4. Boundary sedimentation Biophysical Tools '02 - Sedimentation

  5. Application: Homogeneity Biophysical Tools '02 - Sedimentation

  6. Application: Molecular weight determination • Sedimentation Equilibrium • Sedimentation at low speeds so that the sedimentation is counterbalanced by diffusion Biophysical Tools '02 - Sedimentation

  7. Application: Shape determination Stokes 6.2 S 5.4 S 6.7 S Biophysical Tools '02 - Sedimentation

  8. Application: Purification Biophysical Tools '02 - Sedimentation

  9. Zonal - Density Centrifugation Biophysical Tools '02 - Sedimentation

  10. Practical tips • K-factor: • Pelleting time (t): • But if you spin at speed less than the maximum speed (wmax) k is smaller: • g force Biophysical Tools '02 - Sedimentation

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