LAB SAFETY

1. LAB SAFETY Biology http://www.nclark.net/Biology

2. 1. READ & FOLLOW instructions carefully before attempting to do anything. 2. Wear GOGGLES to protect your eyes from chemicals, heated materials, or things that might be able to shatter. 3. TELL TEACHER if any spills or accidents occur. General Safety Rules

3. 4. After handling chemicals, always WASH YOUR HANDS with soap and water. 5. During lab work, keep your HANDS away from your face. 6. Tie back LONG hair. General Safety Rules

4. 7. Roll up LONG sleeves. 8. Know the LOCATION of the fire extinguisher, fire blanket, eyewash station, and first aid kit. 9. Keep your work area UNCLUTTERED. Take to the lab station only what is necessary. General Safety Rules

5. 10. It is suggested that you wear GLASSES rather than contact lenses. 11. Never put anything into yourMOUTH during a lab experiment. 12. CLEAN your lab area at the conclusion of the laboratory period. 13. NEVER“horse around” or play practical jokes in the laboratory. General Safety Rules

6. 1. Chipped or cracked GLASSWARE should not be used. Show it to the teacher. 2. BROKEN glassware should not be disposed of in a classroom trashcan. There is a special glassdisposal container for it. Use BROOM & DUSTPAN Glassware Safety

7. 1. Wear protective GLOVES and a lab apron whenever heating or pouring hazardous chemicals. 2. Never MIX two chemicals together unless the teacher directs you to. Chemical Safety

8. 3. If you need to smell the odor of a chemical, WAFT the fumes toward your nose with one hand. Do not put your nose over the container and inhale the fumes. Chemical Safety

9. 4. Follow the INSTRUCTIONS of your teacher when disposing of all chemicals. 5. WASH your hands after handling hazardous chemicals. Chemical Safety

10. 1. Lay POWER cords where no one can trip on them or get caught in them. 2. Be sure your hands and your lab area are dry before using electrical equipment. 3. Never poke anything into electrical SOCKETS. Electrical Safety

11. 4. Unplug cords by pulling the PLUG and not the cord. 5. Unplug all electrical equipment at the END of the lab period. Electrical Safety

12. 1. Let burners and hotplates cool down before touching them. Perform the SIZZLETEST to see if they are cool enough. 2. Use tongs and/or protective GLOVES to handle hot objects. 3. Never reach across an OPEN flame or burner. Heating Safety

13. Injury: Burns What To Do: Immediately flush with COLD water until burning sensation is lessened. First Aid

14. Injury: Cuts, bruises What To Do: Do not touch an open wound without safety gloves. Pressing directly on minor cuts will STOP bleeding in a few minutes. Apply cold compress to bruises to reduce swelling. First Aid

15. Injury: Fainting To Do: Provide FRESH air and have the person recline so that their head is LOWERthan the rest of their body. First Aid

16. Injury: Eyes What To Do: Flush eyes immediately with plenty of water (15 MINUTES at the eyewash). If a foreign object is lodged in the eye, do not allow the eye to be rubbed. First Aid

17. Injury: Poisoning What To Do: Find out what substance was responsible for the poisoning and alert the TEACHER immediately. First Aid

18. FIRE SAFETY http://www.youtube.com/watch?v=BLjoWjCrDqg

19. Fires can either be extinguished by smothering the flames with a fire blanket or clothing OR a fire extinguisher! To use a fire extinguisher properly REMEMBER: P-A-S-S! PULL thePIN AIM at theBASE SQUEEZEthe Trigger SWEEPside to side FIRE SAFETY

20. Always use the right type of fire extinguisher for each specific type of fire. *LHHS extinguishers (ABC type) CLASS A Fires: • Fires of organic matter that will leave ashes • Example: WOOD, paper, tissues

21. CLASS B FIRES: • Fires of flammable LIQUIDS • Examples: Gasoline, solvents NOTE: • Water is not a good extinguisher because it spreads the liquid around.

22. CLASS C FIRES: • Fires of an ELECTRICAL nature • EXAMPLES: Computers, photocopiers

23. WHMISWorkplace Hazardous Materials Information Systems The 3 components to the WHMIS system are: • MSDS • Worker Education • Labels on all Products

24. MSDSMaterial SAFETY Data Sheet • Stored in binders in lab areas • All known chemicals • 8 sections (name, ingredients, physical data, fire hazards, reactivity data, toxicological properties, preventive measures, first aid measures)

25. WHMIS Labels – Workplace Hazardous Materials Information System • Compressed GAS ex. Helium, propane • Flammable/Combustible ex: methane gas, acetone • Oxidizing Material ex: Chromic Acid • Poisonous Infectious (IMMEDIATE & Serious Toxic Effects) ex: cyanide • Poisonous Infectious (Other Toxic Effects) ex: mercury, lead • Biohazardous Infectious ex: NEEDLES • Corrosive Materials ex: degreaser • Dangerously Reactive ex: pure sodium

26. HHPS SymbolsHazardous Household Product Symbols • HOUSEHOLD! • Levels of Severity (Danger, Warning and Caution)

27. Precautions related to Dissections: • When working with preserved specimens be in a well-ventilated area • Wear vinyl gloves at all times • To reduce the irritating effects of chemical preservatives, rinse specimen under running water to eliminate excess and dilute what remains • Review procedure for cuts and punctures from dissecting tools • When cleaning dissecting instruments, hold sharp edges away from you. • Always ask the teacher re: proper disposal of specimens…special containers for sharps, animal tissue and preservative disposal

28. When working with bacteriologic specimens: • If you have open wounds or cuts on your hands, cover them with a bandage or wear latex gloves. • Disinfect lab bench tops before and after handling bacterial cultures. • While handling bacteria, keep your hands away from your eyes, nose, and mouth. • Wash your hands thoroughly after handling bacterial • If a culture is spilled, cover the spill area with paper towel and flood the paper towels with disinfectant. Wait 20 minutes and then wipe up the spill with more paper towels and dispose of the paper towel in a empty bag.