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vha-3: Vacuolar-type Proton Translocating ATPase

vha-3: Vacuolar-type Proton Translocating ATPase. Sameera Kongara. Clones. BLAST Results. BLAST Results. Vha-3. 161 amino acids; codes for Subunit c of Vo domain Protein 100% identical to Vha-2 Expressed in gastrointestinal, hypodermal and excretory cells

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vha-3: Vacuolar-type Proton Translocating ATPase

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  1. vha-3: Vacuolar-type Proton Translocating ATPase Sameera Kongara

  2. Clones

  3. BLAST Results

  4. BLAST Results

  5. Vha-3 • 161 amino acids; codes for Subunit c of Vo domain • Protein 100% identical to Vha-2 • Expressed in gastrointestinal, hypodermal and excretory cells • Required for oogenesis, Expression of vha-3 not for embryogenesis

  6. V-type ATPases • Present in vacuoles, endosomes and Golgi • Contains 2 major domains: Vo (membrane bound) and V1 (catalytic) • Vo :Glu residue on Subunit c required • Regulated by dissociation of Vo and V1

  7. Some Important Functions of V-ATPases • Bone remodeling • Receptor- Mediated Endocytosis: Cholesterol metabolism • Immune System: Macrophages

  8. V-type ATPases in Osteoclasts • Osteoclasts use ATPases to remodel bones • Antibiotic Bafilomycin appears to bind to Subunit c to inhibit V- ATPase

  9. Cholesterol metabolism • Acidic environment necessary for dissociation of receptors from ligand

  10. V-ATPases in Cancer • Cellular acidification triggers apoptosis • Extracellular environment more acidic in tumor cells • EXPRESSION OF V-ATPase IN TUMORS • V-ATPases overexpressed in plasma membranes of highly metastatic tumors • pH difference can be used for effective drug delivery

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