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EyeTech Particle Size & Shape Analyzer

EyeTech Particle Size & Shape Analyzer is a versatile instrument that accurately measures particle size and shape using laser technology. It overcomes the limitations of conventional techniques and provides robust and reliable results. The analyzer offers both laser channel for particle size and concentration measurement, as well as a video channel for dynamic shape analysis.

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EyeTech Particle Size & Shape Analyzer

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  1. EyeTech Particle Size & Shape Analyzer

  2. AMBIVALUE BV D max D min What is the size of a particle? Size Related Properties : V = Volume W = Weight S = Surface Area A = Projected Area R = Sedimentation Rate Conventional techniques derive particle size from a (physical) property. Usually Particle Size is expressed as the diameter of a sphere with similar property -> equivalent sphere

  3. AMBIVALUE BV Equivalent Sphere These diameters are fundamentally different for the same particle. The equivalent diameters differ for any non-spherical material. Thus, every PSA technique yields different results for non-spherical materials

  4. AMBIVALUE BV Conventional PSA Techniques The measurement of particle properties has several drawbacks: • Sphere is an extreme particle shape, not reflecting real particles • Significant errors for non-spherical particles • Particle size results dependent on used technique • Instrument response requires calibration • Particle size analysis limited to the measured property ‘Laser Obscuration Time’ measurement solves these drawbacks

  5. AMBIVALUE BV EyeTech - Particles Analyzer Laser Channel: Particle Size & Concentration

  6. AMBIVALUE BV EyeTech Measurement Principle Single Instrument – Dual Measurement Channels

  7. AMBIVALUE BV EyeTech Measurement Principle The EyeTech Laser uses the proven optical bench

  8. AMBIVALUE BV A B C D E F Scanning Laser V Time Ta Tb Tc Td Te Tf EyeTech Measurement Principle The rotating laser beam scans single particles within its focus. The diameter of the particle is directly correlated to the duration of the obscuration. The principle measurement is a time interval, not an intensity measurement. The time domain is independent of particles optical or physical properties

  9. AMBIVALUE BV EyeTech Measurement Principle From the duration of the obscuration (t) and the known rotation velocity of the laser beam (v) the particle diameter (D) can be calculated: D = v . t In relation to the high speed of the rotating laser, the particles are stationary. So, particle movement does not effect particle size measurement. No requirement for pre-knowledge of: • - Refractive index • Temperature • Viscosity variation • Electrical conductivity

  10. Pulse Profile On-Center Off-Center EyeTech Pulse Analysis;Off-Center rejections Pulse Transition Pulse Transition Derivative Profile The Derivative Pulse Width of an On-Center pulse transition corresponds to the laser beam width

  11. Amplitude EyeTech Pulse Analysis; Derivative Pulse Signal Minimum width and maximum amplitude indicate on-center interactions. For accepted particle crossings, the angle between the particle boundaries and the laser beam trajectory approaches 90°.

  12. EyeTech Pulse Analysis; Out-of-Focus rejections Focused Laser Beam Pulse Profile Derivative Pulse Derivative Pulses too wide; Out-Of-Focus pulses are rejected

  13. EyeTech Pulse Analysis Concept; Transparent Particles Opaque Particles Translucent Particles Transparent Particles Pulse Profile Particle Size Measurement is independent of particle’s transparency

  14. AMBIVALUE BV EyeTech Measurement Principle Advantages: • Data is collected on single particles • Detection of minor fractions • Direct measurement of correct particle size, not influenced by physical or optical properties • Operation in liquid, dry or slide • No calibration required • Excellent traceability to (BCR/NIST) standards • Robust measurement, no alignment required by user • Concentration measurement

  15. AMBIVALUEBV FDA SCIENCE POSTER FEB 2001COMPARATIVE ANALYSIS OF COMMON PARTICLE SIZING TECHNIQUES FOR PHARMACEUTICAL POWDERSH.R. Prasanna, E.H. Jefferson, J.S. Taylor, A.S. Hussain, R.F. Karuhn, R.C. Lyon In the FDA poster presented in Feb 2001 they concluded that: “The large population of fine particles was only detected by the particle counting techniques (image analysis and light obscuration) and not by the ensemble technique (laser diffraction)”

  16. Laserdiffraction • Fast & repeatable…. But good? • Assembling technology…general approach. • Assumptions…. Spherical particles, calibration • Restrictions….. Concentration, minor fraction, T • pre-knowledge of sample required… RI (mixture?, unknown sample?), AI (color), Transparency, mirror like (scattering, metals)

  17. AMBIVALUE BV EyeTech - Particles Analyzer Video Channel: Dynamic Shape Analysis

  18. AMBIVALUE BV Why Dynamic Shape Analysis • Conventional particle sizing techniques will always give an equivalent spherical diameter in Number or Volume. • The only information given is a SIZE information expressed in Number or Volume. • Sometimes, these techniques are not sensitive enough to discriminate subtle variations.

  19. AMBIVALUE BV Reasons for Dynamic Shape Analysis • Seeing is believing, See your particles. • Better characterization of your materials. • High resolution data. • Detection and quantification of shape influences. • Validation technique for the laser channel or other particle size equipment. • Number distribution, more sensitive for detection of minor fractions of fines or agglomerates. • Identification of the homogenity of the material • Characterisation of shape differences.

  20. EyeTech Measurement Principle Single Instrument – Dual Measurement Channels AMBIVALUE BV

  21. AMBIVALUE BV EyeTech Video Channel • Video Channel collects digital images used for: • Visualization during laser measurement • Dynamic Shape Analysis • Grabbing Images and Recording Movie

  22. AMBIVALUE BV Seeing is believing • No difference in particle size distributions for different materials • With Dynamic Image Analysis, the difference is evident

  23. AMBIVALUE BV Quantifying of shape differences • The difference in the shape factor and maximum diameter are obvious! Max. Ferret Diameter: Shape Factor:

  24. AMBIVALUE BV Dynamic Shape Analysis Real time images of particles in motion are collected Images are converted into a grid containing a collection of picture elements (pixels) Each individual pixel has a value for brightness (grey level): 0 = Dark; 255 = White All images are analyzed according to a pre-defined set of Image Analysis characteristics Measurement is finished when the measurement end condition is met (# of particles/images, confidence level, time) 420 µm Image Conversion 18 µm

  25. Image Analysis TermsBasic Image Analysis terms:Macro Size Descriptors Ferret Diameters • Determination of distributions of: • Minimum Ferret • Maximum Ferret • Average Ferret Average Ferret diameter: F1 + F2 + F3 + F4 … F36 36 For a sphere size = diameter

  26. Shape Analysis TermsBasic Image Analysis terms:Macro Shape Descriptors [ min Ferret diam.] Aspect Ratio = -------------------------- [ max Ferret diam.] [Area * 4 ] Shape Factor = ------------------ (Circularity) [Perimeter]2

  27. DSA Parameters Comparing Shape Factor to Aspect Ratio Shape Factor Aspect Ratio 1 1 <0,1 1 0,785 0,707 0,604 0,577 0,436 0,250 0,160 0,100

  28. Object Data Base • The complete shape information for every particle is saved in the object data base. • Particles can be viewed individually

  29. AMBIVALUE BV EyeTech Shape Analysis Features: • Easy setup and measurement using software wizards • Advanced pre-processing algorithms • Size and Shape grouping • Automated report generation • Storage of real raw data (un-processed images) • Re-processing of raw data • Calibration and verification tools available • Interface with 3rd party hardware (e.g. microscopes)

  30. AMBIVALUE BV Dynamic Shape Analysis • For each individual particle, size and shape parameters are determined. • The EyeTech Image Analysis software automates set-up and measurement. • EyeTech software includes many IA procedures such as: • Pre-processing procedures • Image Filters • Region of Interest • Out-of-focus Rejection • Morphology Operations • Grouping according to size/shape • (Re-) processing of stored images and movies • Lens Calibration

  31. AMBIVALUE BV Grouping / Shape Filters All: • Shape Filters can be set up to analyse a specific group of particles based on size and/or shape • Appications: • Fibre Analysis • Shape of Sieve Fraction • Abrasives Shape Filter Shape Factor < 0,8: Average Size of non-spherical particles in sample

  32. AMBIVALUE BV Dynamic Shape Analysis DSA provides a solution for characterization of non-spherical materials: • Multiple size & shape parameters to adapt to any application. • No assumptions about particle’s sphericity. • Random orientation of particles. No preferred orientation as in microscopy. • Grouping of particles based upon size or shape. • Visualization for measurement validation/verification.

  33. AMBIVALUE BV Dynamic Shape Analysis Summary • Advanced analysis of video images enables the determination of different shape parameters. • Feret Diameters, Shape Factor and Aspect Ratio for shape characterization. • Isolation of classified particles using strategic Shape Filter options. • Validation of particle size results: “SEEING IS BELIEVING” • More accurate characterization of non-spherical materials • Re-processing of stored data

  34. AMBIVALUE BV Eyetech Analysis Package New Features: • Easy setup and measurement using software wizards • Automated Report Generator Results stored in SQL database • Multiple User Levels • Hardware Diagnostics • Calibration and verification tools available • 21 CFR Part 11 Compliant

  35. AMBIVALUE BV EyeTech User Interface • Laser and video in one program • One click measurement • Real time feedback • Multiple data display of graphs, tables, statistics • On-Line cycle compare • On-Line help • Wizard driven software

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  39. AMBIVALUE BV Setup Method • Setup wizards for laser and video measurements • Step-by-step guidance through the whole setup process • All created methods are stored • Methods can be linked to a sample, user or customer • Load a method to start the measurement

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  42. AMBIVALUE BV Advanced Data Output • Result Database supports: • Data mining: search, sort… • Image retrieval • Networking: import \ export data, images… • Archiving, back up • Measurement cycles comparison • Host mode: Remote data analysis • 21 CFR compliant version includes: • Secure log-in • Electronic signatures • Audit trails

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  45. Object Data Base • The complete shape information for every particle is saved in the object data base. • Particles can be viewed individually

  46. AMBIVALUE BV EyeTech S/W Advantages • Easy setup and measurement using software wizards • Advanced pre-processing algorithmes • Size and Shape grouping • Automated report generator • Storage of real raw data (un processed images) • Re-processing of raw data (movie files) • Interface with third party hardware ( e.g. microscope) • Calibration and verification tools avaible • Concentration measurement • Integrated laser and video software • Simultaneous laser and video results • Pharmaceutical FDA 21 CFR Part 11 Compliant

  47. AMBIVALUE BV One System = Variety of Applications Magnetic Stirring Fiber Cell Free Fall Heated Cell Micro Flow Slide Cell Aerosol Flow Liquid Flow(10mm) Liquid Flow (4mm) Mechanical Stirring “Adapting the technology to the application rather then adapting the application to the technology”

  48. AMBIVALUE BV Unique & useful features… (1) • True multi-tasking technology - multiple tasks enable during measurement • Time based analysis - not depending on any optical properties of the sample • Measurements applicable for a broad of concentration range - no dilution needed • Possibility of kinetics measurements (Crystal growth, dissolution properties, etc) • Investigation of flow ability - process conditions simulations • Particle of interest in large matrix can be characterized selectively by using shape filters • No alignment or calibration needed - not even when changing system compounds • Advanced overflow protection in the automatic Liquid Flow Controller (EyeFlow) • Advanced data base - single particle can be detect - clear & fast internal search engine

  49. AMBIVALUE BV Unique & useful features… (2) • High resolution & accuracy of dynamic measurement range for multi disperse samples • No need for pre-knowledge of sample properties • Ideal for non-spherical particles analysis • Small volume samples - allowing sample volume down to 2 ml • IQ, OQ, PQ validation support available • 21 CFR part 11 compliant S/W • Operation in harsh conditions - minimum maintenance requirements • Easy access, maintenance and cleaning procedure to all system compounds • Robust measurement - independent to laser intensity and no fluctuation adjustment • Emergency measurement stop available at any time by the operator • Particles size distribution based on measuring individual particles - not on ensembles • Simple & easy to explain measurement principle • Fast and easy preparation of samples and user controlled presentation of analysis data

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