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See why.

See why. Massive Analysis of cDNA Ends: MACE. How it works. cDNA population bound to Streptavidine. AAAAAAA-3’ TTTTTTT-5’. 5’ 3’. cDNA. Streptavidin-Beads. 5’ 3’. AAAAAAA-3’ TTTTTTT-5’. cDNA. 5’ 3’. AAAAAAA-3’ TTTTTTT-5’. cDNA. AAAAAAA-3’ TTTTTTT-5’. 5’ 3’.

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See why.

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  1. See why.

  2. Massive Analysis of cDNA Ends: MACE How it works cDNA population bound to Streptavidine AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA Streptavidin-Beads 5’ 3’ AAAAAAA-3’ TTTTTTT-5’ cDNA 5’ 3’ AAAAAAA-3’ TTTTTTT-5’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA AAAAAAA-3’ TTTTTTT-5’ 5’ 3’ cDNA

  3. Massive Analysis of cDNA Ends: MACE How it works Fragmentation AAAAAAA-3’ TTTTTTT-5’ Streptavidin-Beads AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ 100-200 bp

  4. Massive Analysis of cDNA Ends: MACE How it works 2nd generation sequencing of 50-100 bp AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’

  5. Massive Analysis of cDNA Ends: MACE How it works 2nd generation sequencing of 50-100 bp AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’ AAAAAAA-3’ TTTTTTT-5’

  6. Massive Analysis of cDNA Ends: MACE How it works Counting, mapping, assembly, SNP detection 1 1 4

  7. MACE AT CG AT AAAAAAAA TTTTTTTT cDNA Reads SNPs withenoughcoverage: 2 RNA-Seq AT CG AT AAAAAAAA TTTTTTTT cDNA SNPs withenoughcoverage: 0 Reads

  8. Massive Analysis of cDNA ends: MACE SNP-Analysis SNP Rattus norvegicus, laboratory strain, ClustalX view of alpha actin MACE tags

  9. High resolution: rare transcripts included! Transcript-species copy-numbers and distribution MACE Microarray

  10. Massive Analysis of cDNA Ends: MACE Advantages • High-resolution: • only one specific tag is sequenced per transcript • even low-level expressed transcripts are analyzed • High SNP-coverage: • only the highly polymorphic 3’ ends are sequenced • high coverage for reliable SNP detection is warranted • High throughput & low costs: • hundreds of samples can be analyzed simultaneously • multiplexed sequencing reduces costs while still providing high resolution

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