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HEK alone

S1. C/EBP a and E6AP co-localize in the nucleus. HEK alone. HEK+C/EBP α. C/EBP α + E6AP 1.0 μ g. C/EBP α + E6AP 2.0 μ g. Merge. DAPI. E6AP. C/EBP α. S2. GST-E6AP Purification. GST-E6AP+IPTG. GST alone. GST-E6AP. Marker. 1 2 µg(BSA). GST-E6AP. BSA. GST. S3a.

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HEK alone

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  1. S1. C/EBPa and E6AP co-localize in the nucleus HEK alone HEK+C/EBPα C/EBPα+ E6AP 1.0μg C/EBPα+ E6AP 2.0μg Merge DAPI E6AP C/EBPα

  2. S2. GST-E6AP Purification GST-E6AP+IPTG GST alone GST-E6AP Marker 1 2 µg(BSA) GST-E6AP BSA GST

  3. S3a. K562 1 2 3 4 5 6 clone K562 p42C/EBPα-ER p42C/EBPα-ER 67 kDa β actin K562+p42C/EBPα-ER clone2 HEK +p42C/EBPα HEK alone K562 c. b. 67 kDa 42kDa K562-p42C/EBPα -ER clone2 5µM E2 , 5days K562-p42C/EBPα-ER clone2

  4. Fig. S4: E6AP is expressed in various myeloid leukemia cell lines HEK293T+C/EBPα+E6AP HEK293T alone Kasumi1 U937 K562 HL60 E6AP 100kDa β actin

  5. Fig. S5: siE6AP efficiently down regulates E6AP expression K562+siRNA E6AP MCF7+siRNA E6AP HEK+E6AP HEKalone K562 MCF7 100kDa E6AP β actin

  6. Fig. S6: Ectopically expressed C/EBPα physically interacts with E6AP in K562 cells IP E6AP K562 alone K562 alone C/EBPα+ MG132 C/EBPα+ MG132 C/EBPα C/EBPα IP IgG E6AP 100kDa C/EBPα 42kDa

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