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Mdm4

13: MDA-MB-175VII. 11: MDA-MB-134VI. 18: MDA-MB-435S. 36: SUM1315M02. 12: MDA-MB-157. 14: MDA-MB-231. 15: MDA-MB-330. 16: MDA-MB-361. 17: MDA-MB-415. 19: MDA-MB-436. 20: MDA-MB-453. 21: MDA-MB-468. 34: SUM225CWN. 30: SUM149PT. 31: SUM159PT. 32: SUM185PE. 33: SUM190PT. 35: SUM229PE.

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Mdm4

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  1. 13: MDA-MB-175VII 11: MDA-MB-134VI 18: MDA-MB-435S 36: SUM1315M02 12: MDA-MB-157 14: MDA-MB-231 15: MDA-MB-330 16: MDA-MB-361 17: MDA-MB-415 19: MDA-MB-436 20: MDA-MB-453 21: MDA-MB-468 34: SUM225CWN 30: SUM149PT 31: SUM159PT 32: SUM185PE 33: SUM190PT 35: SUM229PE 38: UACC-812 39: UACC-893 23B: OCUB-M 27: SUM44PE 28: SUM52PE 41: ZR75-30 24: SK-BR-3 25: SK-BR-5 26: SK-BR-7 8: HCC1937 22: MPE600 40: ZR75-1 6: DU4475 5: CAMA-1 10: MCF-7 9: Hs578T 7: EVSA-T 37: T47D 2: BT474 4: BT549 1: BT20 Mdm4 Mdm2 ATM p300 CBP HAUSP pRB p53 CDK4 Cyclin D1 PUMA p21 p16 p14ARF Tubulin Tropomysosin Supplementary Figure 1

  2. A B *** *** ** *** *** *** *** *** *** *** ** C D *** * ** *** *** * *** *** *** * *** ** *** *** Supplementary Figure 2

  3. A B C D Supplementary Figure 3

  4. + 10 µM Nutlin-3 Doxorubicin Mdm2 Mdm4 pSer15-p53 pSer46-p53 p53 PUMA p21 HAUSP Supplementary Figure 4

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