1 / 10

Arterieel endothelium

Fibreus weefsel. Vasculaire gladde spier. Arterieel endothelium. MAPCellen and vasculaire ziektes. Arterieel endothelium? Vasculaire gladde spier? Bioartificiele arterie? In vivo therapie voor bvb. gangreen?. TGF- b 1 +/- PDGF-BB induces functional (v)SMCs from MAPCellen. I (pA/pF).

aderyn
Download Presentation

Arterieel endothelium

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Fibreus weefsel Vasculaire gladde spier Arterieel endothelium

  2. MAPCellen and vasculaire ziektes Arterieel endothelium? Vasculaire gladde spier? Bioartificiele arterie? In vivo therapie voor bvb. gangreen?

  3. TGF-b1 +/- PDGF-BB induces functional (v)SMCs from MAPCellen I (pA/pF) RAOSMC rMAPC I (pA/pF) Passage 1 Day 0 Day 2 Day 6 SM22 SM22a Pass 1 TGF-b1 + PDGF-BB SMaActin I (pA/pF) F-rMAPC Day 6 Pass 1 SMaActin RAOSMC SM22 IgG Specific antibody JEFF ROSS, J Clin Invest 2006

  4. MAPCellen and vaatziektes Arterieel endothelium: JA Vasculaire gladde spier: JA Bioartificiele arterie? In vivo therapie voor bvb. gangreen?

  5. Week 5 Rat Aorta MAPCel-gladde spier kan “contractiele” tube vormen Week 5, no mech. cond. MAPC-SMCs + fibrin gel into tubular cavity Cells are entrapped in “gel” formed around a nonadhesive mandrel Cell-induced gel contraction around mandrel CollagenNucleiFibrin JEFF ROSS, JCI 2006

  6. Conditioneren van de tube om contractiliteit te verhogen MAPC-SMC ring constructs are subjected to cyclic distention for 3 weeks at 5% strain, 0.5 Hz, and 12.5% duty cycle JEFF ROSS, JCI 2006

  7. ET-1 ET-1 10 mg 5 min 30 mg 5 min MAPC-SMC Mechanical MAPC-SMC Static MAPCel-gladde spier tube kan samentrekken en relaxeren zoals arterieel bloedvat Traces of MAPC-SMC rings 60 mM KCl 60 mM KCl 50 mg Rho-Kinase inhibitor relaxation 5 min 10 mM ACh 60 mM KCl 10 mM Y-27632 MAPC-SMC Static MAPC-SMC Mechanical Rho-Kinase Inhibitor MAPC-SMC Mechanical Vasodilation JEFF ROSS, JCI 2006

  8. Arterie? • Moet kunnen druk van ~1,000mmHg weerstaan; Momenteel ~300mmHg • Hoe creeren we endothelium aan binnenzijde? • Heel waarschijnlijk niet goed gehecht, en laat loss door “sheer” stress van bloed • Moeten ervoor zorgen dat er geen klonters ontstaan

  9. MAPCellen and vasculaire ziektes Arterieel endothelium: JA Vasculaire gladde spier: JA Bioartificiele arterie? NOG NIET In vivo therapie voor bvb. gangreen?

  10. Perifeer ischemie model Acute Ischemie: Femorale arterie geligeerd Onmiddelijke behandeling “Chronische” Ischemie; ~ klassieke perifere vasculaire insufficientie Iliacale arterie geligeerd Behandeling 5 dagen later extensieve celdood, fibrose en verlies van lidmaat Minimale celdood Luttun et al J Clin Invest In Press

More Related