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A.Konovalov

A.Konovalov NANOASSCIATES ARE THE MATERIAL CARRIES OF THE BIO-EFFECTS MANIFESTED BY HIGH DILUTED WATER SOLUTIONS OF BAC A.Arbuzov Institute of Organic and Physical Chemistry of Kazan Scientific Center of Russian Academy of Sciences, konovalov@knc .ru WATER CONFERENCE 2013 BULGARIA.

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A.Konovalov

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  1. A.Konovalov NANOASSCIATES ARE THE MATERIAL CARRIES OF THE BIO-EFFECTS MANIFESTED BY HIGH DILUTED WATER SOLUTIONS OF BAC A.ArbuzovInstitute of Organic and Physical Chemistry of Kazan Scientific Center of Russian Academy of Sciences, konovalov@knc.ru WATER CONFERENCE 2013 BULGARIA

  2. DEDICATED TO TWENTY FIVE ANNIVERSARY OF PUBLICATION J.BENVENISTE ARTICLE IN “NATURE” (1988)

  3. Facts that are not explained by existing theories are the most valuable for science asfar as exactlyfrom their study we should expect its further development in the near future. A.Butlerov, 1879

  4. MANIFESTATION OF BIOLOGICAL ACTIVITY (BIOEFFECTS) BY HIGH DILUTED WATER SOLUTIONS (HDWS) MAY BE CONSIDERED AS SUCH FACTS THERE ARE THOUSANDS EXAMPLES OF THIS PHENOMENON AND THEY ARE KNOWN MORE THAN HUNDRED YEARS BUT FOR RECENTLY TIME THERE WAS NOT SATISFACTORY EXPLANATION OF SUCH FACTS WE GUESS THAT MATERIAL CARRIES OF THIS PHENOMENON ARE NANOASSOCIATES

  5. A.I.Konovalov Formation of the Nano-sized Moleculare Ensambles in High Diluted Aqueous Solutions. Effect of Ultra-low Concentrations and Electromagnetic Fields. A.E.Arbuzov Institute of Organic and Physical Chemistry of RAS, Kazan The VIIth Annual Conference on The Physics, Chemistry and Biology of Water» October 18-21, 2012, West Dover, Vermont, USA 6 6

  6. FORMATION OF NANOASSOCIATES IS THE KEY TOTHE UNDERSTSNDING OF THE BEHAVIOR OF HIGH DILUTED AQUEOUS SOLUTIONS

  7. CHRONICLE OF OUR WORK • Beginning of investigations 2006 • First oral communication 2007 • First publications on DLS 2008- • and physicochemical study -2009 • Using of term of • «NANOASSOCIATES»2009 • First pulication on the influence • of electromagnetic fields 2011

  8. NANOASSOCIATES are nanosized (up to 400 nm) molecular ensembles, which are formed in high diluted water solutions (HDWS) of BAC under influence of two effectors: solutes and external (natural) electromagnetic fields – and consist of water molecules mainly (up to 500 millions). Formation of nanoassociates designate both physicochemical and ( it is very important) biological properties of HDWS

  9. ALMOST HUNDRED COMPOUNDS WERE STUDIED BY SEVERAL METHODS IN 10-2 – 10-20 M CONCENTRATION INTERVAL

  10. ANTIOXIDANTS PLANT GROWTH REGULATORS NEUROMEDIATORS VITAMINS HORMONES ANTICEPTICS ANXIOLITICS COMPOUNDS WITH KNOWN AND UNKNOWN BIOLOGICAL PROPERTIES

  11. DLS STUDY Phenozan potassium salt

  12. ζ – potencial, mV Phenozanpotassiumsalt

  13. MELAFEN

  14. χ,µS/cm «non-classic behavior»

  15. SOLUTIONS NANOOBJECTS χ , σ … D,ζ CORRELATIONS BETWEEN PARAMETRS OF NANOOBJECTS AND PHYSICOCHEMICAL PROPERTIES OF HDWS EXIST

  16. PHENOZAN POTASSIUM SALT: ζ-potencial of nanoassociates and electrical conductivity of solutions

  17. VIP-1 ICHFAN C-10: ζpotencial of nanoassociates and electrical conductivity of solutions R=СH2CH2N+(CH3)2C10H21Br - 18 18

  18. NANOASSOCIATES сreate «THE WEATHER» IN HDWS

  19. NO SOLUTES NO NANOASSOCIATES SOLUTES ARE EFFECTORS OF NANOASSOCIATES FORMATION BUT NOT ONLY IT NEEDS EM FIELDS ALSO

  20. VIP-2 PHENOZAN POTASSIUM SALT NO NANOASSOCIATES 1-Laboratory table 2-Permalloy container • magnetic induction (В) • of geomagnetic field • in Kazan is 53·103nTl, • in container 10-20 nТl 21 21 21

  21. NO SOLUTES NO NANOASSOCIATES NO EM FIELDS NO NANOASSOCIATES ! Transformation of «non-classic behavior» into «classic behavior» of solutions

  22. NOT ALL SOLUTES FORM NANOASSOCIATES ~ 25% SOLUTES DO NOT FORM NANOASSOCIATES

  23. CMC = 7 ·10-5M AMPHIPHILIC PHOSPHACUMARINE (APC) NO NANOASSOCIATES «classic behavior» 24 24

  24. PHENOZAN POTASSIUM SALT NO NANOASSOCIATES t.c t.c 1-Laboratory table 2-Permalloy container t.c 25 25 25

  25. NO EM FIELDS NO NANOASSOCIATES NO BIOEFFECTS ?

  26. Mal’tseva E., Kasparov V., Pal’mina N. 1-Laboratory table 2-Permalloy container VIP-3 The concentration dependence in the membrane lipid microviscosity of synaptosomeusing HDWS of PhK,фиксируемой методом ЭПР с помощью стабильного свободного радикала 16-доксилстеариновой кислоты, нитроксильный фрагмент которой локализован на глубине 22-26 Αо,

  27. COMPARISON OF PHYSICOCHEMICAL AND BIOLGICAL PROPERTIES OF HDWS OBTAINED IN INDEPENDENT EXPERIMENTS

  28. PHENOZAN POTASSIUM t.c

  29. PHENOZAN POTASSIUM

  30. NO EM FIELDS NO NANOASSOCIATES NO BIOEFFECTS !

  31. NANOASSOCIATES ARE MATERIAL CARRIES OF HDWS BIOEFFECTS !

  32. Mal’tseva E., Kasparov V., Pal’mina N. 1-Laboratory table 2-Permalloy container The concentration dependence of change in the membrane lipid microviscosity of synaptosomeusing DSWS of PhK,фиксируемой методом ЭПР с помощью стабильного свободного радикала 16-доксилстеариновой кислоты, нитроксильный фрагмент которой локализован на глубине 22-26 Αо,

  33. MORE DEEP INVESTIGATION OF HDWS BIOLOGICAL ACTIVITY IN CONNECTION WITH NANOASSOCIATES FORMATION

  34. N.M.EmanuelInstituteofBiochemical Physics of RAS N.Pal’mina team I.Zhigacheva team

  35. Changing of sign of bio-effects of BAC by dilution of solutions under influence of nanoassociates formation

  36. GUANIBIPHOS

  37. GUANIBIPHOS: THE INFLUENCE ON THE DEVELOPMENT OF MICROORGANISMS _ + t.c

  38. ACETYLHYDRAZIDE OF TIACALIX[4]ARENE: INFLUENCE ON THE GROWTH P.BACILLUS SPP - Bacillis subtillis 6633 - Bacillis subtillis L2 t.c

  39. ACETYLHYDRAZIDE OF TIACALIX[4]ARENE: INFLUENCE ON THECONSUPTION OF OXYGEN BY CUTTED OFF ROOTS OF WHEAT t.c

  40. DERIVATIVE OF CALIX[4]RESORCINE

  41. DERIVATIVE OF CALIX[4]RESORCINE: INFLUENCE ON THE CONSUPTION OF OXYGEN BY CUTTED OFF ROOTS OF WHEAT + + _ t.c Концентрационная зависимость биоэффекта, в качестве которого взято потребление кислорода отсеченными корнями пшеницы к 5-му часу инкубации в растворе КРА по отношению к контролю (раствор, не содержащий КРА).

  42. + + _

  43. t.c

  44. «At presentit is evident that remidies can be active in ultra-low doses and these effects in their trends can differ from effects by therapevtic doses.» М.: ФИЗМАТЛИТ, 2010.- 624с.

  45. IBUPROPHENE – FEBRIFUGE 10-14 – 10-10 М – стимулирующее воздействие 10-10 – 10-7 М - не влияет 10-7 – 10-4 М – ингибирующее воздействие Влияние бруфена в широком диапазоне концентраций на высвобождение [3H]-метаболитов арахидоновой кислоты перитониальными макрофагами мыши. За 100 % - отсутствие бруфена Шимановский Н.Л., Епинетов М.А., Мельников М.Я. Молекулярная и нанофармакология. - М.: ФИЗМАТЛИТ, 2010. - 624с.

  46. THE NECESSITY OF THE STUDY OF ALL REMIDIES IN WIDE INTERVAL OF CONCENTRATIONS INCLUDING HDWS

  47. Mal’tseva E., Kasparov V., Pal’mina N. 1-Laboratory table 2-Permalloy container The concentration dependence in the membrane lipid microviscosity of synaptosomeusing DSWS of PhK,фиксируемой методом ЭПР с помощью стабильного свободного радикала 16-доксилстеариновой кислоты, нитроксильный фрагмент которой локализован на глубине 22-26 Αо,

  48. t.c The concentration dependence of the changes inD of nanoassociates (1) and in the membrane lipid microviscosity of synaptosome (2) using HDWS of PhK in hypoelectromagnetic conditions

  49. CONCENTRATION DEPENDENCE OF SIZE DISTRIBUTION IN SOLUTIONS OF CDAzF S. Samal, K. E. Geckeler // Chem. Commun. – 2001. – P. 2224-2225 M. Sedla´k // J. Phys. Chem. B. – 2006. – No. 110. – P. 4329-4338 M. Sedla´k // /J. Phys. Chem. B. – 2006. – No. 110. – P. 4339-4345 M. Sedla´k // J. Phys. Chem. B. – 2006. – No. 110. – P. 13976-13984 L.O. Kononov, N.N. Malysheva, A.V. Orlova // Eur. J. Org. Chem. – 2012. – P. 1926–1934

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