30pts

1. The Arristaless-like (Arl) gene below carries a homeodomain DNA binding domain (HD). Furthermore, it has a proven activator domain (A) and repressor domain (R). Possible WT mRNAs with A-HD, which have activator-like properties as a protein, promote activation of target genes. Possible WT mRNAs with R-HD, which have repressor-like properties as a protein, inhibit activation of target genes. 30pts pA site Arl HD R A E1 E2 E4 E3 SD SD SA Do not express TxFactors E1, E2, E3 or E4 means enhancer binding site Express TxFactors E1 RSS sites 30 cells in WT Antennal Tissue Express TxFactor E1, E2, SA LoxP site Express TxFactors E1, E2, E3 RBPa binding site Express TxFactors E1, E2, E3, E4 (where RBP would bind in the transcribed RNA) Remember that CreERT2 functions Only when Tamoxifen is present -Arl expression is normally dependent on all four TxFactors (E1, E2, E3, and E4) being present. However, if E4 is absent in the DNA, then Arl can also be expressed in cells with both E1, E2 or with E1, E2 and E3. -Rag 1 is expressed only in cells expressing both TxFactors E1 and E2. -Rag 2 is expressed only in cells expressing all three TxFactors: E1, E2 and E3. -A transgene exist in the genome for CreERT2 , but can be only activated by cells expressing AHD protein.-RNA binding protein alpha inhibits splicing at HD. -RNAa is only expressed in cells expressing both E1, E2.

2. EXAM version A Quiz 11: Biol 203 Fall 2013 All questions are 5pts each Name:_______________ In which cells will DNA rearangement occur? In which cells will CreERT2 be expressed? Instructor Name _______________ In which cells will DNA be deleted in the presence of Tamoxifen (addition of Tamoxifen is only after cells have formed)? In which cells is Arl expressed prior to RSS mediated recombination? In which cells will unspliced mRNA be made for Arl? In which cells can Cre mediated recombination and RSS recombination BOTH occur in the genome of a cell?