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Chapter One. Research Methods in Physiological Psychology. Histology Autopsy/ Lesion Imaging/ Recording. Stimulation Pharmacological methods Genetic methods. Diverse Research Methods Are Used in Biological Psychology. Histology : The Study of Microscopic Structures and Tissues.
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Chapter One Research Methods in Physiological Psychology
Histology Autopsy/ Lesion Imaging/ Recording Stimulation Pharmacological methods Genetic methods Diverse Research Methods Are Used in Biological Psychology
Histology: The Study of Microscopic Structures and Tissues • Tissue to be viewed must be: • fixed by freezing or formalin. • sliced thinly by a microtome. • Stains are applied to highlight structures of interest
Histology Examples Myelin Stain Golgi Stain Nissl Stain
Using Histology: Horseradish Peroxidase axon of neuron neuron synapse Normal flow of information Thalamus Retina Retrograde Transport
Lesions • The results of lesions can be used to determine the function of an area. • Lesions may be: • naturally occurring • artificially produced • heating the tips of surgically implanted electrodes • chemicals that kill cell bodies. • temporarily produced by cooling an area of the brain.
Brain Imaging Example - Brain Activity • Positron Emission Tomography (PET) • Measures brain activity based on the utilization of radioactive glucose or oxygen. Courtesy Dept. of Energy Office of Public Affairs
Stimulation Delgado uses electrical stimulation to control his fighting bull. From Jose Delgado “Ratbots” respond to stimulation by changing direction.
Pharmacological Methods • Drugs: • Administered to subject, behavior/brain activity monitored • Microdialysis: • Chemical samples are removed through micropipettes. • Identify chemicals present in a very small area.
Genetic Methods • Twin Studies • Monozygotic (identical) vs. dizygotic (fraternal) twins • Concordance rates • Genetically Modified Animals • Knockout genes
Human Ethical Guidelines • No coercion • Informed consent • No harm • Confidentiality
Non-Human Animal Ethical Guidelines • Clear purpose and necessity • Excellent housing, food and health care • Minimal pain and suffering
Research Ethics Oversight • Federal guidelines • Professional societies recommendations by (APA, Society for Neuroscience) • Institutional review boards • Human Subjects Committee • IACUC • Journals