10 likes | 128 Views
A. 10 20 30 40 50 60 70 80 90 ....|....| ....| ....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....|
E N D
A 10 20 30 40 50 60 70 80 90 ....|....| ....| ....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| B(LAI)TCCGGAGTAC TTCAAGA.... .......... .........A CTGCTGACAT CGAGCTTGCT ACAA...GGG ACTTTCCGCT GGGGACTTT X ---------- -A---AG.... .......... .........- -------TGA A--AG--T-- -AC...T--- ---------- --------- B1 ------A--- -A---AG.... .......... .........- ----C----- ----.--T--- ---..G--- ---------- --------- B2_S ------A--T -A---AG.... .......... .........- ---------- ----.--T--- ---..G--- ---------- --------- B2_L --------TT -A-----ACTG CTGACGTCGA GTTACAGGG- ---------- ---A---T-CG --...G--- ---------- --------- AE ------A--- -AT--AG.... .......... .........- ---------A A--AG--T--- AC...TAA- ----.----- --------- 100 110 120 130 140 150 160 170 180 ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| ....|....| B(LAI)CCA.GGGAGG CGTGGCCTGG GCGGGACT.G GGGAGTGGCG AGCCCTCAGA TCCTGCATAT AAGCAGCTGC TTTTTGCCTG TACTGGGTCT X ---.------ T--TA----- -----GT-.- ---------T -A-------- -G-------- -------C-- ----C--T-- ---------- B1 ---A------ T--------- --------.- ---------- ---------- -G-------- ---------- ---------- ---------- B2_S ---A------ T--------- --------.- ---------- ---------- -G-------- ---------- ----.----- ---------- B2_L ---.-A---- ----A-A--- --------.- ---------- -A-------- -G-------- ---------- ----.----- ---------- AE ---G------ T------G-- -T--AGT-.- --------TT -A-------- -G-------A -------C-- ----C--T-- ---------- 190 200 210 220 230 240 ....|....| ....|....| ....|....| ....|....| ....|....|....|....| ... B(LAI)CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAACCCACTGCTT AAG X ----A----- ---------- ---------- ---------- -G------------------ --- B1 ---------- --------A- ---------- ---------- G------------------- --- B2_S ---------- --------A- ---------- ---------- G------------------- --- B2_L ---------- ---------- ---------- ---------- -G------------------ --- AE ----T----- -----G--.- ----C----- ---------- -G-A---------------- --- BseAI TATAA-136 RBE III NF-κβII NF-κβI GABP AP1 SP1III SP1II SP1I CATATAA-30 E +1 BfrI CACCC-bi B C B (LAI) AE B1 B2_S B2_L X G G G G G G G G G G U U U U U G G G G G A A A A A C C C C C G G U G U U A C A A C C C C C U U U U U U U U A A A A A A C C C C C G C ∆G = -29.6 ∆G = -29.9 ∆G = -29.7 ∆G = -29.6 ∆G = -29.0 C ∆G = -29.7 C G U A U U U U C G U G G G A A U U G G C A G G G C G U C G U C A A G U G C C A A C U C G C G G G G C A G G C U G C C G U C A G C A U A C C A G U G U C U C U G G U U A G C U U U C U U C G C G C A G A C G A G G U G C A G G C C U G U U C G C U G G G A U U U C C A C G G A A A G C G U U U G G A U U C U A G C C C G G G U U C G U A G G G G U C C C A A C G C G A C A G G G C G A U G C C G U A C A G U G C U U G C C G U U U A G U G G C C C C A A A C A G C C U G C C U U U C A G G A G A A G G C G C A A U C G U G G C C C A C G U U G G A C G G U G G G G C U G C C C U C A U A U A