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SGN Database. TOM1 Array. Use all spot positions (e.g. 1.2.3.4) to query SGN database, for Unigenes. Predicted protein sequences. Unigene List
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SGN Database TOM1 Array Use all spot positions (e.g. 1.2.3.4) to query SGN database, for Unigenes Predicted protein sequences Unigene List >sgn|U225498 Lycopersicon Combined #3 [5 ESTs aligned] arabidopsis/peptide: At1g67090.2 68414.m07630 ribulose bisphosphate carboxylase small chain ... genbank/nr: gi|3914578|sp|O22573|RBS3_FRIAG Ribulose bisphosphate carboxylase small chain 3, chloroplast precursor (RuBisCO small subunit 3) ... >sgn|U225499 Lycopersicon Combined #3 [6 ESTs aligned] arabidopsis/peptide: At1g42960.1 68408.m04528 expressed protein (evalue: 3.2e-28, score=121.7) genbank/nr: gi|18400762|ref|NP_564482.1| expressed protein [Arabidopsis thaliana] gi|25373224|pir||B96497 >sgn|U225500 Lycopersicon Combined #3 [35 ESTs aligned] arabidopsis/peptide: At1g69410.1 68408.m07313 Eukaryotic initiation factor 5A -related similar to eukaryotic initiation factor 5A genbank/nr: gi|20138708|sp|Q9AXQ6|IF51_LYCES Eukaryotic translation initiation factor 5A-1... TCCCAGTAGTCCCA..... ... INTERPRO Domains Unigene List, containing i) Arabidopsis blast search result ii) genebank/nr blast search result ii) Sequence of the unigne INPARANOID Sequences were used to blast against latest Arabidopsis Protein models Genebank nr hits used for manual classifications Possible Arabidopsis orthologs and in-paralogs Classification of Unigenes represented on the TOM1 array
SGN Database TOM1 Array Query SGN database with all spot Postions (e.g. 1.2.3.4) seperately to obtain 5‘ unigene and 3‘ unigene Unigene corresponding to 3‘ read Unigene corresponding to 5‘ read Classification of Unigenes represented on the TOM1 array Spot Classification based on 5‘ read Combine results Spot Classification based on 3‘ read Classification of Spots represented on the TOM1 array