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Transcription: DNA mRNA. Remember…. DNA is the genetic code and is found in the nucleus . The genetic code is used to make proteins , which you are made of. But…proteins are made on the ribosomes. Remember…. nucleus. DNA. So how does the code get from the nucleus to the ribosomes?.
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Remember… • DNA is the genetic code and is found in the nucleus. • The genetic code is used to make proteins, which you are made of. • But…proteins are made on the ribosomes.
Remember… nucleus DNA
So how does the code get from the nucleus to the ribosomes? RNA
Structure of RNA • RNA differs from DNA in 3 ways: • RNA is a single strand. • RNA has the sugar ribose. • RNA has the nitrogen base uracil instead of thymine.
Structure of RNA Single strand
3 Types of RNA • Messenger RNA (mRNA) • Ribosomal RNA (rRNA) • Transfer RNA (tRNA)
mRNA • mRNA delivers the instructions for making a protein from the nucleus to a ribosomein the cytoplasm.
Transcription • DNA cannot leave the nucleus. • However, its code can be copied by mRNA and taken out of the nucleus.
Transcription • First, the enzyme RNA polymerase binds to DNA and separates the DNA strands.
Transcription • Then, a strand of mRNA is transcribed (copied) using one DNA strand as a template.
Transcription • To copy the code, RNA nucleotides are added using the base pairing rules for DNA, except that in RNA, uracil pairs with adenine. • (G – C, A – U)
Practice Transcribing • Let’s practice transcribing a strand of DNA: • DNA: G T C A G A T A C • mRNA:________________ C A G U C U A U G
Transcription Animation • http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf
RNA Processing • The sequences that code for proteins are called exons because they are “expressed” in the synthesis of proteins. • The introns do not code for proteins and are cut out of the mRNA sequence before it leaves the nucleus. • The remaining exons are spliced together.
After Transcription • The single strand of mRNA leaves the nucleus and enters the cytoplasm. • It will then travel to a ribosome where its code will be translated into a protein.
Transcription & Translation Transcription Translation In the nucleus In the cytoplasm (ribosome)