TEM-1A * 5’-GAGTATTCAACATTTCCGTGTC-3’ TEM-1B * 5’-TAATCAGTGAGGCACCTATCTC-3’

5. GAATTCACTAGTGATTGGGAATTCTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA CATTCAAATATGTATCCGCTCATGATACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGA GTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGC TCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAG CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTT TTAAAGTTCTGCTATGTGGTGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCAT ACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGA TCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACCCGCCTTGATCGTTG GGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACA ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGG AGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATC TGGAGCCGGTGAGCGTGGATCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTG CCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCGGATCCCAATCGAATTCCCGCGGCC

6. TEM-1A* 5’-GAGTATTCAACATTTCCGTGTC-3’ TEM-1B* 5’-TAATCAGTGAGGCACCTATCTC-3’ SHV-1A 5’-TGGTTATGCGTTATATTCGCC-3’ SHV-1B 5’-GCTTAGCGTTGCCAGTGCT-3’ PER-1A 5’-ATGAATGTCATTATAAAAG-3’ PER-1B 5’-AATTTGGGCTTAGGGCAGAA-3’ VEB-1A 5’-CGACTTCCATTTCCCGATGC-3’ VEB-1B 5’-GGACTCTGCAACAAATGCGC-3’ OXA-10A 5’-GTCTTTCGAGTACGGCATTA-3’ OXA-10B 5’-ATTTTCTTAGCGGCAACTTAC-3’ OXA-23A 5’-GATGTGTCATAGTATTCGTCG-3’ OXA-23B 5’-TCACAACAACTAAAAGCACTG-3’ OXA-24A 5’-TTCCCCTAACATGAATTTGT-3’ OXA-24B 5’-GTACTAATCAAAGTTGTGAA-3’

8. Analysis primers

10. 145

11. 992 992-144=848bp

12. Design and analysis primers