When it comes to chromatography, you must choose the best instrument

1. When it comes to chromatography, you must choose the best instrument Adsorption chromatography is a technique for separating compounds based on the difference in affinity between these compounds, the mobile phase, which drives the compounds, and the stationary phase. Introduction The technique presented here is thin layer chromatography (TLC). It uses a stationary phase deposited on an aluminum plate. The mobile phase is driven by capillarity up the plate. This technique is very widely used, especially when making columns, as an analysis technique. The chromatography supplies of the equipment must be of higher quality to study the result. During this sequence, you will see the principle of this technique on two examples - the separation of dyes visible to the naked eye and the separation of non-colored compounds that will have to be revealed by another means.

2. Realization of the experiment Once the deposit is made, simply place the plate in the tank and observe the rise of the solvent front. When the solvent front reaches two centimeters from the top of the plate, it is removed and immediately marked by a line this forehead, to indicate where the eluent has arrived. This trait is called the solvent front. Chromatography equipment is essential to understand the readings from the examination. Without a proper instrument, you cannot make such progress. This is why it is suggested to buy only higher quality materials. Classification The chromatographic plate generally consists of two parts - a layer of about 0.25 mm of silica gel, fixed to a rigid plate of aluminum, plastic or sometimes glass. Fixation is provided by a binder which may be hydrated calcium sulfate, starch or an organic polymer. The absorbent paper allows saturating the chromatography vials with eluent vapor, which will prevent the eluent on the plate from evaporating and thus disrupt the chromatographic analysis. Source URL: https://bit.ly/2ITZO3k