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Immunological Methods in Microbiological Testing

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Immunological Methods in Microbiological Testing

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    1. Immunological Methods in Microbiological Testing

    3. Antigen: any “thing”, foreign to the immune system. e.g. bacteria, viruses, (or their parts), pollen, etc.

    4. Antibody: proteins produced by the immune system which help defend against antigens

    5. Antiglobulins: antibodies produced by one species against antibodies of another

    6. Immune system: a quick overview

    7. Polyclonal vs monoclonal antibiodies Polyclonal antibodies derived from multiple B-cell lines A mixture of immunoglobulin molecules secreted against a specific antigen, each recognising a different epitope Monoclonal identical to each other Clones of a single parental cell

    8. Immunological Techniques: Background

    9. Immunological Techniques: Background

    10. Immunological Techniques: Background

    11. Immunological Techniques: Background

    12. Immunological Techniques: Background

    13. Fluorescent Radioisotopes Iodine Enzymes Immunological Techniques: Labels

    14. Immunological Techniques: ELISA

    15. Immunological Techniques: ELISA

    16. Immunological Techniques: ELISA

    17. Immunological Techniques: ELISA

    18. Immunological Techniques: Immuno-Magnetic Separation

    19. Very fast In line technology. Flow cytometry (fluorescently labeled bacteria) Kits are commercially available (your techs don’t have to bleed mice and goats!) Very specific to a particular antigen. Very sensitive ELISA plates are suitable for field work Immunological Techniques: Advantages

    20. Some protocols require enrichment. Slows you down. In line technology. Flow cytometry. Requires expensive equipment Kits are commercially available, but not cheap Microscopes may be necessarily Very specific to a particular antigen. Won’t recognize any other antigen False positives/negatives possible, especially with mutated/altered antigen Immunological Techniques: Disadvantages

    21. Western Blot

    22. Western Blot

    23. Affinity Chromatography

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