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Strawberry Soilborne Diseases: Diagnostics, Research

Strawberry Soilborne Diseases: Diagnostics, Research. Steven Koike University of California Cooperative Extension. Macrophomina: July 26. Fusarium wilt. 2006. 2006. Macrophomina. Fusarium wilt. 2015. 2015. Macrophomina. Fusarium. Macrophomina. Fusarium. Macrophomina. Diagnosis:

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Strawberry Soilborne Diseases: Diagnostics, Research

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  1. Strawberry Soilborne Diseases:Diagnostics, Research Steven Koike University of California Cooperative Extension

  2. Macrophomina: July 26

  3. Fusarium wilt 2006 2006 Macrophomina

  4. Fusarium wilt 2015 2015 Macrophomina

  5. Fusarium Macrophomina Fusarium Macrophomina

  6. Diagnosis: Macrophomina Fusarium Verticillium Phytophthora ? ?

  7. Diagnostic challenge: similar symptoms SymptomsMac.Fus.VerticilliumPhytoph. Poor growth yes yes yes yes Stunting yes yes yes yes Dieback yes yes yes yes Pl. collapse yes yes yes yes Crown discolor yes yes no yes Stress yes yes yes no Assoc. w/ H2O no no no yes

  8. Expanding UC diagnostic services • New DNA-based testing for soil path. • Recombinase polymerase amplification • Rapid, species specific detection • Phytophthora (now) • Verticillium • Fusarium • Macrophomina • Acknowledgements: CSC, Frank Martin, Tim Miles, Alyssa Burkhardt, Stacy Mauzey

  9. RPA

  10. +

  11. Root inoculation (sclerotia; 24/30 C) Root Foliar M. phaseolina Plantnecrosissymptomsre-isolated Bean no no (no)* Corn no no no Cotton no no no Soybean no no no Sunflower no no (no)* Watermelon no no no Strawberry yes yes yes Straw. (ctrl) no no no * No necrosis; 1 positive re-isolation/50 root pieces.

  12. Sunflower Corn 24 C 30 C Cotton 24 C 30 C 24 C 30 C

  13. Sunflower Soybean

  14. Crown inoculation with infested toothpicks

  15. Crown inoculation (toothpick; 24 C) Crown Collapse M. phaseolina Hostnecrosissymptomsre-isolated Bean no no no Corn no no no Cotton no no no (Pea) … … … Pepper no no no Potato no no no Sunflower no no no Tomato no no no Strawberry yes yes yes Straw. (ctrl) no no no

  16. Fusarium wilt Cultivar trial Pathogen population Fumigation trial Tom Gordon Department of Plant Pathology

  17. Test for resistance to Fusarium wilt

  18. 2016 test of susceptibility to Fusarium wilt 5 4 3 Disease severity 2 1 Albion Portola Festival Ventana Grenada Petaluma Radiance Fronteras Monterey Sweet Ann San Andreas

  19. Resistant San Andreas Fronteras Festival Portula Ventana Moderately resistant Petaluma Susceptible Grenada Radiance Albion Monterey Highly susceptible Sweet Ann

  20. UC Davis screening trial Most strawberry genotypes fall into one of two groups: susceptible or resistant

  21. Colonization versus resistance Percentage of root tips infected

  22. Colonization versus resistance Percentage of root tips infected San Andreas 34.0 ± 3.6% Albion 36.5 ± 4.1%

  23. Extent of colonization Cultivar Crown Petiole Runner Albion + + + San Andreas +- - Fronteras + - -

  24. F. o. fragariae Three strains in CA F. o. fragariae F. o. fragariae

  25. Do F. o. fragariae strains differ in virulence? Genotype * strain test Genotype Albion Benicia Fronteras San Andreas 5.165 Strain GL 1315 GL 1381 AMP 132

  26. Genotype * strain test 5 4 3 Disease severity 2 1 No differential interaction

  27. Fumigation trial at Monterey Bay Academy 75 lbs. Pic chlor 60 150 lbs. Pic chlor 60 300 lbs. Pic chlor 60 75 lbs. AITC + 1,3 – D 150 lbs. AITC + 1,3 – D 300 lbs. AITC + 1,3 – D

  28. Fumigation trial at MBA 300 lbs. Pic Chlor 60 75 lbs. Pic Chlor 60

  29. F. o. fragariae Colony forming units per gram Control 75 lbs Pic chlor 60 300 lbs Pic chlor 60 150 lbs Pic chlor 60 150 lbs AITC + 1,3 - D 75 lbs AITC + 1,3 - D 300 lbs AITC + 1,3 - D Treatment

  30. F. oxysporum Colony forming units per gram Treatment

  31. Correlation between F. o. fragariae and F. oxysporum F. o. fragariae Fusarium oxysporum

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