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SCREENING OF VARIABLES. COMPARISON OF DIRECT VERSUS INDIRECT LABELING. SCREENING OF VARIABLES. FMDV (+). RT-PCR. P. dsDNA. Lambda Exonuclease. ssDNA FMDV (-). Alexa 647. Labeled ssDNA FMDV(-). Hybridization. PERFECT MATCH (PM). MISMATCH (MM). SCREENING OF TEMPERATURE.
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SCREENING OF VARIABLES COMPARISON OF DIRECT VERSUS INDIRECT LABELING SCREENING OF VARIABLES FMDV (+) RT-PCR P dsDNA Lambda Exonuclease ssDNA FMDV (-) Alexa 647 Labeled ssDNA FMDV(-) Hybridization PERFECT MATCH (PM) MISMATCH (MM) SCREENING OF TEMPERATURE Washing PM MM Scanning Fig.3