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Studi di immunogenicità in modelli murini Laura Pisapia Giovanna Del Pozzo

Studi di immunogenicità in modelli murini Laura Pisapia Giovanna Del Pozzo. Napoli, 27 Giugno 2013.

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Studi di immunogenicità in modelli murini Laura Pisapia Giovanna Del Pozzo

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  1. Studi di immunogenicità in modelli murini Laura Pisapia Giovanna Del Pozzo Napoli, 27 Giugno 2013

  2. CorsoC. et al. EBP1 and DRBP76/NF90 bindingproteins are included in the Major HistocompatibilityComplexClass II RNA operon. 2011 N.A.RSep1;39(16):7263-75.Pisapia L. et al. Co-regulatedexpressionofalpha and beta mRNAsencoding HLA-DR surfaceheterodimersismediatedby the MHCII RNA operon. 2013 N.A.R. Apr1;41(6):3772-86. MHC class II RNA Operon

  3. Identified as ErbB3 binding protein, is the human homologue of mouse cell-cycle regulated protein p38-2G4 Transcriptional repressor: Inhibits transcription of E2F regulated promoters by recruiting HDAC2/Rb/Sin3A complex • RNA binding protein: • influences bcl2 stability, promotes decay of androgen receptor mRNA, • binds UTRs of MHCII EBP1 • Translational control: • in the cytoplasm is associated to 80S ribosomes and polysomes • inhibits translation of AR mRNA • In carcinoma cell lines p48 isoform functions as tumor suppressor • In PC12 and glioblastoma p48 works as oncogene, while p42 isoform is a tumor suppressor p48 and p42 isoforms

  4. Over-expression of p42 and p48 EBP1 influences MHCII amount • p48 isoformbinds 5’ and 3’UTR of MHCII mRNAs • The over expression of p48 isoformdetermines an up- regulation of MHC Class II in differentcancercelllines

  5. OT-II MOUSE OT-I MOUSE Spleen Spleen T CD4+ magneticselection T CD8+ magneticselection CD4+ CFSE labelling CD8+ CFSE labelling B16-OVA and CD4+ co-culture B16-OVAa and CD8+ co-culture T cells activation and proliferation are demonstrated by the decrease of CFSE labelling CD4 OVA323–339 (ISQAVHAAHAEINEAGR) peptide CD8+ T cell CD8 OVA257–264 (SIINFEKL) peptide CD4+ T cell Melanoma B16-OVA CFSE

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