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Aromatase and Kryptolebias marmoratus by J. Newsome. http://www.itrainsfishes.net/content/biotopes/mangrove.php. Background. Aromatase is an enzyme critical in sexual determination Converts androgen to estrogen Conserved across all vertebrates and mammals
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Aromatase and Kryptolebiasmarmoratusby J. Newsome http://www.itrainsfishes.net/content/biotopes/mangrove.php
Background • Aromatase is an enzyme critical in sexual determination • Converts androgen to estrogen • Conserved across all vertebrates and mammals • Located in the brain, ovary, and testes • This study assesses protein alignment and evolutionary relationships between Kmar and other Teleosts
Materials and Methods • Obtained sequence from NCBI • Taxonomy report • Saved in FASTA format • Put sequences in SEAVIEW • Converted to Protein • Aligned Protein Sequence • Used RaxML • Determined Bootstrap values • Input into Figtree • Graphical representation of evolutionary relationship
Results Protein Alignment
Results Aromatase Tree
Results Concatenated Tree
Conclusion • Similar protein structure when Kmar compared to other Teleosts • Teleosts evolved at similar rates • Kmar and Medaka closely related • Evolved at similar evolutionary rates • Out-group evolved separately