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+ laeA2

A. 2.8 kb. Sca I. Sca I. Probe. SANK 18767. laeA1. Sca I. Hyg r. P pgk. T pgk. laeA1/laeA2. Sca I. pSAK-ExplaeA1/laeA2. Sca I. Sca I. Sca I. Sca I. 2.2 kb. 5.5 kb. Hyg r. Transformant. B. C. + laeA2. + laeA1. + laeA1. + laeA2. M. 4. 3. 2. 1. P. 4. 3. 2. 1.

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+ laeA2

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  1. A 2.8 kb ScaI ScaI Probe SANK 18767 laeA1 ScaI Hygr Ppgk Tpgk laeA1/laeA2 ScaI pSAK-ExplaeA1/laeA2 ScaI ScaI ScaI ScaI 2.2 kb 5.5 kb Hygr Transformant B C + laeA2 + laeA1 +laeA1 +laeA2 M 4 3 2 1 P 4 3 2 1 (kb) 5.5 2.2 D +laeA1 +laeA2 Fig. S3

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