0.1

1. 0.1 12 15 14 85 20* 22* 24 28* 25 23* 88 78 2 79 3 71 19 1* 83 85 18 62 94 53 69 98 17* 99 72 91 100 21 6 26 96 16 4 7 9 11* 8 5* 27 10* 13 Supplementary Figure 1 15. AAY37326 Pseudomonas syringae B728a AAO56097 Pseudomonas syringae DC3000 P43505 Neisseria gonorrhea MtrC CAJ24703 Xanthomonas campestris AAM37349 Xanthomonas campestris pv. citri AAO49780 Pseudomonas putida SepA EAO12292 Xylella fastidiosa AAK69563 Pseudomonas putida TgtT AAD15663 Pseudomonas stutzeri TbtA AAL44019 Agrobacterium tumefaciens c58 AAO57754 Pseudomonas syringae DC3000 AAM35219 Xanthomonas axonopodus pv. citri CAD17970 Ralstonia solanacearum Rsp0819 AAG03814 Pseudomonas aeruginosa PAO1 MexA • CAD13539 Ralstonia solanacearum AcrA • YP_585729 Ralstonia metallidurans AcrA • AAZ62798 Ralstonia eutropha AcrA • YP_406903 Shigella flexneri • NP_289833 E. coli O157:H7 • ABB31051 Geobacter sulfurreducens • YP_621022 Burkholderia cenocepacia • CAH34805 Burkholderia pseudomallei • NP_459471 Salmonella typhimurium • AAQ21215 Erwinia amylovora • BAC77250 Serratia marcescens SdeX • ABA74634 Pseudomonas fluorescens Pf0-1 • NP_768155 Bradyrhizobium japonicum • CAC41580 Mesorhizobium loti

2. 0.1 17 8 Supplementary Figure 2 7* 16* 6* 83 5* 20 100 13* 100 99 19 9* 71 90 99 93 73 8* 92 2* 15* 77 52 10 26 69 53 23 83 34* 88 53 3 67 27 94 29 64 56 12 1 4* 89 56 88 74 84 33 97 21 22 31 14 28 30 24 DinF Family 16. AAQ21212 Erwinia amylovora NorM* AAF85483 Xylella fastidiosa 9a5c norM Q879Z5 Xylella fastidiosa Temecula1 norM AAO53914 Pseudomonas syringae DC3000 MATE family CAD14882 Ralstonia solanacearum GMI1000 RSc1180 ZP_00460454 Burkholderia cenocepacia MatE CAH35378 Burkholderia pseudomallei MatE AAF93268 Vibrio cholerae el tor DinF ZP_00631278 Paracoccus denitrificans MatE ABA78359 Rhodobacter sphaeroides MATE family protein RSY03136 Salmonella typhimurium LT2 Na+ driven MDR RAV01352 Azotobacter vinelandii Na+ MDR RMLO04680 Mesorhizobium loti MAFF303099 Na+ driven MDR RPFL00460 Pseudomonas fluorescens Na+ driven MDR RRPA02598 Rhodopseudomonas palustris Na+ driven MDR RSM04712 Sinorhizobium meliloti Na+ driven MDR RBX03146 Bacteroides fragilis Na+ driven MDR RRLE05610 Rhizobium leguminosarum Na+ driven MDR RPA06399 Pseudomonas aeruginosa PAO1 PmpM RHD05071 Bacillus halodurans Na+ driven MDR 11 25 35 32 • CAD17315 Ralstonia solanacearum “matE” Rsp0164 • BAB68204 Vibrio parahaemolyticus “VmrA” NorM family • CAD18364 Ralstonia solanacearum NorM family RSp1213 • NP_521844 Ralstonia solanacearum DinF • NP_288099 E. coli MATE family NorA/YdhE • BAC59742 Vibrio parahaemolyticus NorM • BAB79260 Vibrio cholerae non-01 VcmA* • NP_188997 Arabidopsis thalianaAT3G23550 MDR • NP_191462 Arabidopsis thalianaAT3G59030 MDR • NP_418468 E. coli K12 dinF • AAL46115 Agrobacterium tumefaciens strain C58 dinF • AAO58273 Pseudomonas syringae DC3000 dinF • AAL00559 Streptococcus pneumoniaedinF • NP_767781 Bradyrhizobium japonicum USDA110 dinF • BAB70470 Vibrio cholerae VcrM*