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About OMICS Group

About OMICS Group.

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About OMICS Group

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  1. About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information on Sciences and technology ‘Open Access’, OMICS Group publishes 400 online open access scholarly journals in all aspects of Science, Engineering, Management and Technology journals. OMICS Group has been instrumental in taking the knowledge on Science & technology to the doorsteps of ordinary men and women. Research Scholars, Students, Libraries, Educational Institutions, Research centers and the industry are main stakeholders that benefitted greatly from this knowledge dissemination. OMICS Group also organizes 300 International conferences annually across the globe, where knowledge transfer takes place through debates, round table discussions, poster presentations, workshops, symposia and exhibitions.

  2. About OMICS Group Conferences OMICS Group International is a pioneer and leading science event organizer, which publishes around 400 open access journals and conducts over 300 Medical, Clinical, Engineering, Life Sciences, Phrama scientific conferences all over the globe annually with the support of more than 1000 scientific associations and 30,000 editorial board members and 3.5 million followers to its credit. OMICS Group has organized 500 conferences, workshops and national symposiums across the major cities including San Francisco, Las Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai.

  3. Aqueous Solubility and Degradation Kinetics of the Phytochemical Anticancer Thymoquinone; Probing the Effects of Solvents, pH and Light JUMAH M. M. SALMANI, Ph.D CHINA PHARMACEUTICAL UNIVERSITY

  4. Background Cancers account for 20-25% of deaths in clinical practices. Attempts to cure or palliate cancer employ 3 basic methods: • Surgery • Radiotherapy • Chemotherapy.

  5. Cancer Chemotherapy It is the use of drugs to inhibit or kill proliferating cancer cells, while leaving host cells unaffected. The optimum achievement of this goal is still far from reality.

  6. Limitations of Chemotherapy

  7. Is there any Alternatives? • Naturally occurring phytochemical compounds with anticancer activity.

  8. Nigella Sativa

  9. Why? • Long History, thousands of years of use in folk medicine. • Readily Available in low cost. • High Safety profile. • Contain many bioactive products among them “Thymoquinone” whose anticancer effects has been recently documented.

  10. Thymoquinone It is the Bioactive component of Nigella Sativa with documented: • Anti-inflammatory • Anti-histaminic • Anti-diabetic • Analgesic • Anticancer

  11. Thymoquinone Anticancer Effects: • TQ exerts its pharmacologic effects through the modulation of the physiological and biochemical events responsible for the generation of ROS. • In normal tissues, TQ behave as a powerful antioxidant. • In cancer tissue it works as Pro-oxidant. • Play regulatory role

  12. Inhibition of cancer Hallmarks

  13. Literature Survey • Hundreds of reports documented the activity of TQ most particularly in cancer therapy mediated by different mechanisms. • It has protective effect for normal tissue. • It has augmenting effect for other anticancer agents. • It can repair the damage caused by other Chemotherapeutics agents. • Yet very few reports about the formulations were found.

  14. Previous Formulations Drawbacks • Low Drug Loading & Encapsulation Efficiency • Burst Release Pattern • No Stability Study

  15. The Goals of current Study • In vitro quantitative analysis of Thymoquinone in order to perform Aqueous Solubility and Stability study and the role of solvents, pH and light is to be elucidated as well.

  16. Quantitative Analysis of Thymoquinone • HPLC • Forced Degradation study • Acid • Base • UV • Oxidation

  17. HPLC Validation as per ICH • Linearity (0.5-10 μg/mL , R2=0.9999) • Specificity • Precision (RSD% < 2%) • LOD and LOQ (0.081 and 0.246 µg/mL )

  18. TQ peak 10 min Main UV degradation peaks Acid degradation peaks Main base Degradation peaks Oxidation degradation peaks

  19. Summary • Our developed HPLC method for detection and quantification of TQ was proved to be as a Stability Indicating Method.

  20. Solubility study • Performed using excess of TQ in different Aqueous solvents for 72 hours in closed dark conditions to exclude the effect of light.

  21. Results of solubility study The values represent the concentration of TQ ± SD in μg/ml

  22. Aqueous Stability Study • TQ in Series of aqueous solvents. • Dark to avoid light • Tightly closed container • fixed temp. • HPLC.

  23. Results of stability study:

  24. Effect of Light • The effect of light was studied for TQ solutions in organicsolvents (in which the drug was shown to be stable for at least 7 days in dark to exclude the effect of solvent). • The study performed in light illumination chamber at 750 lux at 25°C.

  25. Results: • Severe degradation of TQ within 24 h regardless the solvent type. • Kinetics of light degradation of TQ follow 2nd Order. • In absence of light TQ is stable in ethanol and 1:1 ratio ethanol :water/buffer.

  26. Initial TQ chromatogram in ethanol After 48 h subjected to light Initial TQ UV spectrum After 48 subjected to light

  27. Conclusion • TQ aqueous solubility compromised by stability • TQ is unstable in all aqueous solvents with direct effect of pH on the degradation. • In dark TQ stable in ethanol and ethanol: water/buffer mixture of 1:1 ratio. • Light has severe effect on TQ stability. • Stability issue of TQ in aqueous solvents for the first time to be studied, and previous formulation discrepancies may be correlated to this issue.

  28. Upcoming studies (unpublished yet) • Thermodynamic analysis of TQ. • Investigating the role of Physicochemical properties of TQ and the formulation strategies on developing successful formulation. • Finding the solution then carrying out thorough evaluation both in vitro and in vivo.

  29. Latest Findings: • We got promising results that may have a role in the near future clinical use of the valuable drug in cancer therapy.

  30. Thank you

  31. Lets Meet again at Pharmacognosy-2015 3rd International Conference and Exhibition on Pharmacognosy, Phytochemistry and Natural Products October 26-28, 2015 Hyderabad, India Theme: Advanced trends for the future of Herbal Drugs and Products Website:http://pharmacognosy-phytochemistry-natural-products.pharmaceuticalconferences.com/

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