450 likes | 1.9k Views
Fleas and Lice. Veterinary Entomology 208 Spring 2010. Compound Microscope. Magnification Always start at lowest magnification (lowest power objective) Move to higher objective as necessary (3-4 stations only) Focus Adjust width of oculars Adjust coarse, then fine focus
E N D
Fleas and Lice Veterinary Entomology 208 Spring 2010
Compound Microscope Magnification Always start at lowest magnification(lowest power objective) Move to higher objective as necessary (3-4 stations only) Focus Adjust width of oculars Adjust coarse, then fine focus If on the highest magnification, only adjust the fine focus to avoid breaking slide Light Use condenser to adjust amount of light (dark vs. glare) Higher magnifications may require more light
Pictorial and Dichotomous Keys • Be sure you read where the key is sending you next, as it can get confusing (particularly with lice) • Look at arrow on insect to see what characteristic is being studied • The small arrows in the key indicate the structure referenced in the couplet
Fleas (Siphonaptera) • 6 specimens: • Ctenocephalides felis • Leptopsylla segnis • Nosopsyllus fasciatus • Echidnophaga gallinacea • Pulex irritans • Xenopysylla cheopis
Flea Anatomy receptaculum seminis
Ocular bristle Below eye In front of eye
Key • 1st couplet: number of combs • We don’t have a 3 comb flea • 2 comb fleas: • Eye presence/absence will separate these 2 species • 1 comb flea: • There is only one in our set • 0 comb fleas: • Head shape separates out Echidnophagagallinacea • Ocular bristle position separates the other 2 species
Mallophaga • 12 specimens of Mallophaga: • Heterodoxus spiniger • Menacanthus stramineus • Menopon gallinae • Chelopistes meleagridis • Goniodes gigas • Goniocotes gallinae • Goniodes dissimilis • Lipeurus caponis • Felicola subrostrata • Bovicola crassipes • Bovicola bovis • Bovicola limbata
Anoplura • 10 specimens of Anoplura: • Pediculushumanus • Phthirus pubis • Haematopinussuis • Haematopinusasini • Haematopinuseurysternus • Solenopotescaplillatus • Linognathus africanus • Linognathus setosus • Linognathus pedalis • Linognathus vituli