An Introduction For Yeast Expression

1. Yeast Expression Yeast is the simplest eukaryotic expression system. It sustains post-translational modification and proper folding of eukaryotic proteins, while still allows easy culture and scale-up production at relatively low cost. The two most commonly used yeast species are Saccharomyces cerevisiae and Pichia pastoris. Features Disulfide-bonded protein expression Glycosylated protein expression Intracellular/Secreted expression Large-scale eukaryotic protein production at low cost P.pastoris is the most commonly used yeast species for recombinant protein expression. Culture of P.pastoris is simple and inexpensive. It grows fast and can reach high cell density, which supports high yield and large-scale production of eukaryotic proteins. It is also a recommended system for glycosylated or disulfide-bonded proteins. Profacgen designed expression vectors with strong promoters that support either secretory or intracellular production of your target proteins. Pichia pastors is a species of methylotrophic yeast. They have a high growth rate and are able to grow on a simple, inexpensive medium.

2. S.cerevisiae is the best-understood eukaryotic species. Besides the similar advantages as that of P.pastoris, it is also recognized by FDA to be a GRAS (generally regarded as safe) organism. Saccharomyces crevice is used in making wine, bread and beer. They’re characterized by the inability to use nitrate and ability to ferment various carbohydrates. Profacgen provides you with an ultimate solution of yeast production. Our service can be tailored according to your specialized requirements (codon optimization, tag selection, purity, endotoxin removal etc.). Our expertise guarantees high protein quality, short turnaround time, and the best price in the market! See more details on the principle and protocol of yeast expression system on our website. See more...