Spec2go

1. Spec2go™ quick and easy spectroscopy Spec2go 0015.0 ng A portable and affordable sample quantitation device. object shown to scale Precise measurements of concentrations up to 1μg/μl.

2. Spec2go™ Spec2go Closed/Sample Measurement Mode 0015.0 ng 0000.0 ng sample 1 microliter of sample is added to each surface Spec2go Open/Sample Loading Mode

3. Measurement Mode • occurs at a single separation distance 0.0000ng

4. Concentration easily calculated from absorbance measurement Absorbance Sample Concentration All based on the linear relationship between absorbance (what the Spec2go™ physically measures) and concentration (what you want to know).

5. At high concentrations, this linearity breaks down Absorbance Sample Concentration • Caused by: • strong electrostatic interactions between molecules • changes in refractive index • shifts in chemical equilibrium • Higher concentrations require a shorter pathlength for measurement

6. No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising:  [A] first and second anvil surfaces -at least one being moveable relative the other to any one of threepositions;  [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface;  [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces;  [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement.

7. No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising:  [A] first and second anvil surfaces -at least one being moveable relative the other to any one of threepositions;  [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface;  [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces;  [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. The Spec2go™ only has two positions. Loading position Measuring position

8. No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising:  [A] first and second anvil surfaces -at least one being moveable relative the other to any one of threepositions;  [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface;  [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces;  [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. Both surfaces of Spec2go™ are wet at loading position, not in the compression position. The liquid drop on the first surface never wets the opposing surface.

9. No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising:  [A] first and second anvil surfaces -at least one being moveable relative the other to any one of threepositions;  [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface;  [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces;  [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. Samples in the Spec2go™ are not pulled from a compressed state to a measuring state. Loading position Measuring position

10. No Literal Infringement 1. A photometric or spectrophotometric apparatus wherein -a sample in the form of a liquid drop is contained by surface tension forces between two planar surfaces, one containing --a photometric or spectrophotometric source and the other --a photometric or spectrophotometric detector and -an optical path is established through the sample between the two surfaces said apparatus comprising:  [A] first and second anvil surfaces -at least one being moveable relative the other to any one of threepositions;  [B] an adjustable sample loading position so selected that -the at least one moveable surface and -the other surface are so remotely spaced that --a liquid drop can be placed on the first surface;  [C] an adjustable sample compression position so selected that -the surfaces are --opposed and substantially parallel and proximally spaced so that ---the liquid wets and spreads upon both surfaces;  [D] an adjustable sample measuring position so selected that -the opposed substantially parallel surfaces are spaced apart to --pull the sample into a column wherein ---it is contained by the surface tension thereby providing an optical path for a photometric or spectrophotometric measurement. pin fixed plunger The measurement spacing of Spec2go™ is set by a pin and a non-adjustable plunger. Side view of Spec2go™

11. No Infringement Under DOE Claim Element: Three positions

12. No Infringement Under DOE Claim Element: Sample compression position … the liquid [drop] wets … both surfaces

13. No Infringement Under DOE Claim Element: Sample measuring position … pull the sample into a column

14. No Infringement Under DOE Claim Element: Adjustable measuring position