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PROTEIN ASSEMBLY

PROTEIN ASSEMBLY. REVIEW: PROTEIN STRUCTURE. Polypeptide (Protein): Multiple amino acids connected by peptide bonds (polymer). One single amino acid (monomer). PROTEIN SYNTHESIS REVIEW. Central Dogma: DNA->mRNA-> tRNA -> a.a .-> protein Transcription (DNA to mRNA)

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PROTEIN ASSEMBLY

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  1. PROTEIN ASSEMBLY

  2. REVIEW: PROTEIN STRUCTURE Polypeptide (Protein): Multiple amino acids connected by peptide bonds (polymer) One single amino acid (monomer)

  3. PROTEIN SYNTHESIS REVIEW • Central Dogma: DNA->mRNA->tRNA->a.a.->protein • Transcription (DNA to mRNA) and Translation (mRNA-> proteins)

  4. CODONS • 3 Bases long • You must be able to recognize codons! DRAW LINES TO SEPARATE CODONS: AUC|GAC|UAU| • AUG isa START CODON (METHIONINE or MET amino acid)

  5. CODONS continued • Only one start codon (AUG), multiple STOP codons • CODONS code for amino acids. (3 bases rqd. to form a codon) • One or two bases alone mean nothing! • Many codons code for the same amino acid

  6. AMINO ACID CHART • You must know how to use the amino acid chart. • Use the mRNA codon to find the amino acid • Amino acids are abbreviated using first 3 letters: • Serine written as Ser, Arginine as Arg, etc.

  7. Activity: PB pg. 367 Quick Lab • Using the base sequence: GACAAGTCCACAATC • Write sequence on a separate piece of paper. • From left to right write the mRNA transcribed from this gene. • Using the amino acid chart, write the amino acid sequence for the polypeptide • Repeat step 2, reading the sequence of mRNA molecule from right to left. • Answer questions #1 and #2 in the textbook.

  8. EXIT SLIP • Separate the following sequence into codons APPROPRIATELY: ATTGCCATCGAT • When reading an amino acid chart, what do you use? Explain. • What do start and stop codons do?

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