P arameters frefoils called factors in pregnant women

1. Parameters frefoils called factors in pregnant women Ondrova Dana, Svestak Marek, Pilka Radek , Stejskal DavidTDpt of laboratory medicine Central Moravian hospital County, Prostejov, Czech Republic; Dpt of Obsteric anf gynecology Faculty hospital and Dpt of medical chemistry and biochemisty Faculty of medical and dentistry Palacky University Olomouc , Czech Republic OPTIONALLOGO HERE OPTIONALLOGO HERE Objectives Methods Results Conclusions Methods: tested 56 pregnant women (after informed consent to testing), who gave birth to healthy children, 12 pregnant women with suspected aneuploidy. For all pronbands excluding women with aneuploidy was examined during the 1st, 2nd, 3rd Trimester hCG, AFP, E3, hCG-beta and PAPP-A (MOM) and performed repeated ultrasound examination. There was developed sandwich ELISA own method for determining TFF1/2 with satisfactory analytical characteristics. Results: The concentration of TFF1 gene product during pregnancy did not change (median 0.4, 0.5, 0.5, P = 0.56), TFF2 significantly increased (median 3.1, 4, 13, P >0.01). We determined correlation between hCG and TFF1 1st trimester (negat) and positive correlation between hCG and TFF1 in 2nd trimester of gravidity. We did not found correlations between chromosomal aberations (trisomy of 21, 18, 13) and TFF1/2 values. For the first time was done measuring the concentration of TFF1 and TFF2 in pregnant women during pregnancy in the peripheral blood, where it was found that TFF2 during pregnancy significantly increases and can assume its role during pregnancy. TFF1 a TFF have no role in incidence of chromosomal aberations Trefoil family in humans consists of three peptides (TFF-1, -2, -3) which play a role mainly in the repair process of mucosal epithelium in the gastrointestinal tract, but also in the urothelial tract and elsewhere. TFFs is a specific function in copper binding and are characterized by expression in the GIT, pancreas, cartilage, lungs, urinary tract or placenta. TFF expression increases before partum and decreases after. Yet been tested in pregnant protein concentration Objective: To determine the concentration of TFF1 and TFF2 in pregnant women during pregnancy, TFF determinate the relationship with the occurrence of aneuploidy. References Without informations