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Beers Law for a Single Component Sample

Beers Law for a Single Component Sample. b. I 0. I. A = Absorbance = - log 10. I / I 0. I 0 = Incident beam intensity I = Transmitted beam intensity. b = Optical path length c = Solution Concentration (M/L) ε = Molar Absorptivity (L/M cm). Atomic Absorption Spectrometry.

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Beers Law for a Single Component Sample

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  1. Beers Law for a Single Component Sample b I0 I A = Absorbance = - log10 I / I0 I0 = Incident beam intensity I = Transmitted beam intensity b = Optical path length c = Solution Concentration (M/L) ε = Molar Absorptivity (L/M cm)

  2. Atomic Absorption Spectrometry • Advantages over solution U.V./Vis spectrometry • More selective – narrow atomic lines, compared with broad molecular bands Only 5 spectral overlaps known • Lock-and-key match with HCL source and atoms in flame cell • Working ranges from 0.1 – 1,000 ppm

  3. Molecular band absorption Continuum source Typical Molecular absorption band Molecular band overlap area 250 400 200 350 300 Wavelength (nm)

  4. Atomic line absorption Hg 253.7 nm (HCL source) Hg 253.7 nm (atoms in flame) 250 400 200 350 300

  5. Atomic line absorption Atomic line absorption

  6. Helium Arc Lamp spectrum Mercury Arc Lamp spectrum

  7. Typical Atomic Absorption Spectrometer

  8. Electrothermal Vaporization AAS Electrothermal Vaporizer (ETV) Graphite Tube Electrode Terminals

  9. Methods of Sample Introduction in Atomic Spectroscopy

  10. Types of Atomizers Used for Atomic Spectroscopy

  11. Processes that occur in flames atomize ionize

  12. Properties of Flames

  13. Degree of Ionization of Metals at Flame Temperatures

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