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By Paris Thatos

The Effect of Hydra Head Activator on the Regeneration Rates of Heads and Tails of Planaria (Dugesia Tigrina). By Paris Thatos. Need. Only by learning more and more about regeneration can we hope to understand and, one day, reproduce its effects in people. Abstract.

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By Paris Thatos

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  1. The Effect of Hydra Head Activator on the Regeneration Rates of Heads and Tails of Planaria (Dugesia Tigrina) By Paris Thatos

  2. Need • Only by learning more and more about regeneration can we hope to understand and, one day, reproduce its effects in people.

  3. Abstract • This study focuses on the regenerative rates of a species of planaria, and how they could be manipulated through the use of a neuropeptide that is known to influence regeneration in another regenerative species, the freshwater polyp, hydra. Individual planaria were dissected into a head half and a tail half, then were kept in separate Petri-dishes, each with a pre-determined concentration of the neuropepride (Hydra Head Activator). Their rates of regeneration were observed, then graded on the blastema growth using a numerical scale (1 - 7)

  4. Knowledge Base • Planaris were dissected with a razor, and then kept in petri dishes full of the pre-determined concentration of HA • A webcam was used to observe the blastema • A seven stage scale was used to score the data

  5. Hypothesis • H0 - When treated with the HA, planaria heads will regenerate as quickly as those untreated, and tails will instead regenerate heads • Ha - When treated with the HA, planaria heads and tails will not regenerate as quickly as those untreated, tails will regenerate tails

  6. Project Goals • The goal of this study is to find out if the neuropeptides used to influence hydra regeneration will play the same role in planaria regeneration

  7. Methodology Planaria n=12 (Dugesia Tigrina) Treated with Hydra Head Activator 100ppmn=2 10ppmn=2 25ppmn=1 5ppmn=1 12.5ppmn=3 1ppmn=3 Dissected into Head and Tail halves Dissected into Head and Tail halves Dissected into Head and Tail halves Dissected into Head and Tail halves Dissected into Head and Tail halves Dissected into Head and Tail halves Head Tail Head Tail Head Tail Head Tail Head Tail Head Tail Observed with a Webcam and graded based on a seven stage scale

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