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Analyse de microgouttes par mesure d’absorption sur puce microfluidique

Analyse de microgouttes par mesure d’absorption sur puce microfluidique

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Analyse de microgouttes par mesure d’absorption sur puce microfluidique

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  1. Analyse de microgouttes par mesure d’absorption sur puce microfluidique G.Aubrya,b, S. Méancea,b, A-M Haghiri-Gosnetb, Q. Koua,c, aLaboratoire de Photophysique Moléculaire, CNRS, 91405 Orsay, France,bLaboratoire de Photonique et de Nanostructures, Route de Nozay, 91460 Marcoussis, France, cUniversité Paris-Sud, 91405 Orsay, France. Contact : guillaume.aubry1@u-psud.fr Motivations • Microfluidic droplet devices have been created so that small quantity of sample can be encapsulated in droplets produced in dynamic way. Numerous novel applications have emerged based on these capabilities in biology and chemistry. • In this work, we present an original way to perform sensitive absorption measurement using a microfluidic dye laser on volumes as small as a few nanoliters. Experimental Set Up Clean Room Process Material Fluids : gain medium = Rhodamine 6G at 5.10-3 mol/L dispersed phase liquid = water continuous phase liquid = oil Optical pump : doubled frequency Nd:YAG laser, 532nm, 10mW in average, 6KHz pulse frequency Detection system : collection of the signal via a 200µm core diameter multimode fiber and acquisition with a spectrometer (Ocean Optics USB2000+) a) Metallisation of golden fibers b) Photolithography and revelation c) Placement of sacrificial fibers Schematics representation Experimental Results Variation of laser intensity in time as droplets flow through the cavity: before (a), during (b), after (c) d) PDMS deposit Geometrical conditions required for lasing effect Unstable cavity e) Removal of sacrificial fibers Stable cavity Confined droplets are necessary to lase f) Insertion of golden fibers Microphotographs & Laser Spectra a) g) Irreversiblebonding Conclusion & Perspectives • An new system was designed to perform absorption measurement on small volumes : it combines intra-cavity laser system and droplet microdroplet production system. • The experience was performed on nanoliter droplets. Moreover this volume can be reduced, depending on the channel geometries. • Automation of the detection system would allow to proceed to investigate the performances of such a system. JOURNEES NANOMICROTECHNOLOGIES 09

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